Rabbit anti-Human, Mouse APAF1 Polyclonal Antibody | anti-APAF1 antibody

APAF1 Antibody

Cat. Num. AAA9435053

• Gene Names: APAF1; CED4; APAF-1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Peptide affinity purified
• Synonyms: APAF1; Polyclonal Antibody; APAF1 Antibody; APAF 1 antibody; Apaf-1 antibody; APAF_HUMAN antibody; Apaf1 antibody; Apoptotic peptidase activating factor 1 antibody; Apoptotic protease activating factor 1 antibody; Apoptotic protease activating factor antibody; Apoptotic protease-activating factor 1 antibody; CED 4 antibody; CED4 antibody; KIAA0413 antibody; anti-APAF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Peptide affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-APAF1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Peptide
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis on cell lysates using anti-APAF1 rabbit polyclonal antibodies.)

Related Product Information for anti-APAF1 antibody

Apoptotic protease activating factor 1, also known as APAF1, is a human homolog of C. elegans CED-4 gene. This gene encodes a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. This protein contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. The precise mechanism for this reaction is still debated though work published by Guy Salvesen suggests that the apoptosome may induce caspase 9 dimerization and subsequent autocatalysis. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis.

Product Categories/Family for anti-APAF1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4502123
• NCBI GeneID: 317
• NCBI Accession #: NP_001151.1
• NCBI GenBank Nucleotide #: NM_001160.2
• UniProt Accession #: O14727; O43297; Q7Z438; Q9BXZ6; Q9UBZ5; Q9UGN8; Q9UGN9; Q9UGP0; Q9UJ58; Q9UJ59; Q9UJ60; B2RMX8
• Molecular Weight: 141,840 Da
• NCBI Official Full Name: apoptotic protease-activating factor 1 isoform b
• NCBI Official Synonym Full Names: apoptotic peptidase activating factor 1
• NCBI Official Symbol: APAF1
• NCBI Official Synonym Symbols: CED4; APAF-1
• NCBI Protein Information: apoptotic protease-activating factor 1
• UniProt Protein Name: Apoptotic protease-activating factor 1
• Protein Family: Apoptotic protease-activating factor
• UniProt Gene Name: APAF1
• UniProt Synonym Gene Names: KIAA0413; APAF-1
• UniProt Entry Name: APAF_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

APAF: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). By E2F and p53/TP53 in apoptotic neurons. Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 12q23

Cellular Component: cytosol; nucleus; apoptosome

Molecular Function: identical protein binding; protein binding; nucleotide binding; caspase activator activity; ADP binding; ATP binding

Biological Process: regulation of apoptosis; caspase activation; nervous system development; neuron apoptosis; positive regulation of apoptosis; apoptosis; neural tube closure; forebrain development; caspase activation via cytochrome c