Human Wingless Type MMTV Integration Site Family, Member 7A (WNT7A) RTU ELISA Kit | WNT7A rtu elisa kit

Human Wingless Type MMTV Integration Site Family, Member 7A (WNT7A) ELISA Kit

Cat. Num. AAA4503398

• Reactivity: Human
• Synonyms: Wingless Type MMTV Integration Site Family; Member 7A (WNT7A); Human Wingless Type MMTV Integration Site Family; Member 7A (WNT7A) ELISA Kit; Proto-Oncogene Wnt7a Protein; WNT7A rtu elisa kit

• Reactivity: Human
• Sequence Length: 349

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 31.2-2000pg/mL
• Sensitivity: 11.6pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WNT7A rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT7A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to WNT7A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT7A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WNT7A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 17505191
• NCBI GeneID: 7476
• NCBI Accession #: NP_004616.2
• NCBI GenBank Nucleotide #: NM_004625.3
• UniProt Accession #: O00755; Q96H90; Q9Y560
• Molecular Weight: 39,005 Da
• NCBI Official Full Name: protein Wnt-7a
• NCBI Official Synonym Full Names: Wnt family member 7A
• NCBI Official Symbol: WNT7A
• NCBI Protein Information: protein Wnt-7a
• UniProt Protein Name: Protein Wnt-7a
• UniProt Gene Name: WNT7A

NCBI Description

This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. Mutations in this gene are associated with Fuhrmann and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndromes. [provided by RefSeq, Jul 2008]

Uniprot Description

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway (). Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PubMed:16826533). Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes. Required for normal neural stem cell proliferation in the hippocampus dentate gyrus. Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation. Promotes formation of synapses via its interaction with FZD5 ().