Human Protein Wnt-7a ELISA Kit | WNT7A elisa kit

Human Protein Wnt-7a (WNT7A) ELISA Kit

Cat. Num. AAA282281

• Reactivity: Human
• Synonyms: Protein Wnt-7a; Human Protein Wnt-7a (WNT7A) ELISA Kit; WNT7A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human WNT7A. No significant cross-reactivity or interference between Human WNT7A and analogues was observed.
• Sequence Length: 349

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for WNT7A elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WNT7A in samples. An antibody specific for WNT7A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWNT7A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT7A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT7A bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 17505191
• NCBI GeneID: 7476
• NCBI Accession #: NP_004616.2
• NCBI GenBank Nucleotide #: NM_004625.3
• UniProt Accession #: O00755; Q96H90; Q9Y560
• Molecular Weight: 39,005 Da
• NCBI Official Full Name: protein Wnt-7a
• NCBI Official Synonym Full Names: Wnt family member 7A
• NCBI Official Symbol: WNT7A
• NCBI Protein Information: protein Wnt-7a
• UniProt Protein Name: Protein Wnt-7a
• Protein Family: Protein
• UniProt Gene Name: WNT7A

NCBI Description

This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. Mutations in this gene are associated with Fuhrmann and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndromes. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT7A: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts. Defects in WNT7A are the cause of limb pelvis hypoplasia aplasia syndrome (LPHAS). A syndrome of severe deficiency of the extremities due to hypo- or aplasia of one or more long bones of one or more limbs. Pelvic manifestations include hip dislocation, hypoplastic iliac bone and aplastic pubic bones. Thoracic deformity, unusual facies and genitourinary anomalies can be present. Defects in WNT7A are a cause of Fuhrmann syndrome (FUHRS); also known as fibular aplasia or hypoplasia femoral bowing and poly- syn- and oligodactyly. Fuhrmann syndrome is a distinct limb-malformation disorder characterized also by various degrees of limb aplasia/hypoplasia and joint dysplasia. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p25.1

Cellular Component: cell surface; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane; proteinaceous extracellular matrix

Molecular Function: cytokine activity; frizzled binding; protein binding; receptor binding; receptor ligand activity

Biological Process: angiogenesis; asymmetric protein localization; axonogenesis; cartilage condensation; cell fate commitment; cell proliferation in forebrain; central nervous system vasculogenesis; cerebellar granule cell differentiation; chondrocyte differentiation; dorsal/ventral pattern formation; embryonic axis specification; embryonic digit morphogenesis; embryonic forelimb morphogenesis; embryonic hindlimb morphogenesis; establishment of cell polarity; fallopian tube development; negative regulation of apoptosis; negative regulation of neurogenesis; neurotransmitter secretion; palate development; positive regulation of cellular protein metabolic process; positive regulation of epithelial cell proliferation involved in wound healing; positive regulation of JNK cascade; positive regulation of synaptogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of axon diameter; response to estradiol; response to estrogen; satellite cell compartment self-renewal involved in skeletal muscle regeneration; sex differentiation; skeletal muscle satellite cell activation; somatic stem cell division; somatic stem cell maintenance; stem cell development; Wnt receptor signaling pathway through beta-catenin; Wnt signaling pathway

Disease: Fibular Aplasia Or Hypoplasia, Femoral Bowing And Poly-, Syn-, And Oligodactyly; Ulna And Fibula, Absence Of, With Severe Limb Deficiency