Mouse Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ELISA Kit | WNT10B elisa kit

Mouse Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) ELISA Kit

Cat. Num. AAA2020640

• Gene Names: WNT10B; SHFM6; WNT-12
• Reactivity: Mouse
• Synonyms: Wingless Type MMTV Integration Site Family; Member 10B (WNT10B); Mouse Wingless Type MMTV Integration Site Family; Member 10B (WNT10B) ELISA Kit; WNT-12; SHFM6; WNT10B elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 10B (WNT10B).; No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) and analogues was observed.
• Sequence Length: 389

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WNT10B elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 10B (WNT10B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 10B (WNT10B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 10B (WNT10B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 10B (WNT10B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for WNT10B elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 64654936
• NCBI GeneID: 7480
• UniProt Accession #: O00744; O00747; Q4VAJ4; Q4VAJ5; Q8WZ97; B2R7A5
• Molecular Weight: 20,483 Da
• NCBI Official Full Name: Wingless-type MMTV integration site family, member 10B
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 10B
• NCBI Official Symbol: WNT10B
• NCBI Official Synonym Symbols: SHFM6; WNT-12
• NCBI Protein Information: protein Wnt-10b
• UniProt Protein Name: Protein Wnt-10b
• UniProt Gene Name: WNT10B
• UniProt Synonym Gene Names: WNT12
• UniProt Entry Name: WN10B_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT10B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Defects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6). SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: frizzled binding; receptor agonist activity

Biological Process: positive regulation of apoptosis; skeletal muscle fiber development; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; neuron differentiation; positive regulation of cell proliferation; chondrocyte differentiation; positive regulation of epithelial cell differentiation; positive regulation of transcriptional preinitiation complex assembly; cell cycle arrest; G2/M transition of mitotic cell cycle; negative regulation of epithelial cell proliferation; smoothened signaling pathway; Wnt receptor signaling pathway; cell fate commitment; protein stabilization; sensory perception of taste; regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of bone mineralization; negative regulation of fat cell differentiation; positive regulation of osteoblast differentiation; positive regulation of transcription factor activity; regulation of skeletal muscle development; lipid metabolic process; positive regulation of anagen; myoblast cell differentiation involved in skeletal muscle regeneration

Disease: Split-hand/foot Malformation 6