Human Protein Wnt-10b ELISA Kit | WNT10B elisa kit

Human Protein Wnt-10b ELISA Kit

Cat. Num. AAA762580

• Gene Names: WNT10B; SHFM6; WNT-12
• Reactivity: Human
• Synonyms: Protein Wnt-10b; Human Protein Wnt-10b ELISA Kit; WNT10B/Protein Wnt-12/WNT10B/WNT12; WNT10B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of WNT10B. No significant cross-reactivity or interference between WNT10B and analogues was observed.
• Sequence Length: 389

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WNT10B elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 16936522
• NCBI GeneID: 7480
• NCBI Accession #: NP_003385.2
• NCBI GenBank Nucleotide #: NM_003394.3
• Molecular Weight: 20,483 Da
• NCBI Official Full Name: protein Wnt-10b
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family member 10B
• NCBI Official Symbol: WNT10B
• NCBI Official Synonym Symbols: SHFM6; WNT-12
• NCBI Protein Information: protein Wnt-10b
• UniProt Protein Name: Protein Wnt-10b
• Protein Family: Protein
• UniProt Gene Name: WNT10B
• UniProt Synonym Gene Names: WNT12
• UniProt Entry Name: WN10B_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT10B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Defects in WNT10B are the cause of split-hand/foot malformation type 6 (SHFM6). SHFM is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. SHFM6 is a autosomal recessive disorder. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: frizzled binding; receptor agonist activity

Biological Process: cell cycle arrest; cell fate commitment; chondrocyte differentiation; G2/M transition of mitotic cell cycle; lipid metabolic process; myoblast cell differentiation involved in skeletal muscle regeneration; negative regulation of epithelial cell proliferation; negative regulation of fat cell differentiation; negative regulation of transcription from RNA polymerase II promoter; neuron differentiation; positive regulation of anagen; positive regulation of apoptosis; positive regulation of bone mineralization; positive regulation of cell proliferation; positive regulation of epithelial cell differentiation; positive regulation of osteoblast differentiation; positive regulation of transcription factor activity; positive regulation of transcriptional preinitiation complex assembly; protein stabilization; regulation of proteasomal ubiquitin-dependent protein catabolic process; regulation of skeletal muscle development; sensory perception of taste; skeletal muscle fiber development; smoothened signaling pathway; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin

Disease: Split-hand/foot Malformation 6