Wisteria floribunda Lectin
Wisteria floribunda (WFA, Japanese Wisteria) (Agarose)
Applications
ELISA
Purity
Molecular Biology Grade
Synonyms
Wisteria floribunda; Wisteria floribunda (WFA; Japanese Wisteria) (Agarose); Wisteria floribunda lectin
Host
Wisteria floribunda seeds
Purity/Purification
Molecular Biology Grade
Form/Format
Supplied as a liquid in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.1mM Ca++, 20mM galactose, 0.08% sodium azide. Labeled with Agarose.
Applicable Applications for Wisteria floribunda lectin
ELISA (EL/EIA)
Application Notes
Suitable for use in ELISA.
Activity
2x10e7
Binding capacity
4mg N-acetylgalactosaminyl glycoprotein/ml gel
Inhibiting/Eluting Sugar
200mM N-acetylgalactosamine 3mg lectin/ml gel
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Wisteria floribunda lectin
Wisteria Floribunda lectin is a 116,000 to 125,000 molecular weight glycoprotein with an isoelectric point of pH 5.4. Two subunits of about 60,000D are produced in SDS gels, dissociating into 4 chains of about 30,000D in the presence of reducing agents. The binding specificity is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked alpha or beta to the 3 or 6 position of galactose. Used to fractionate lymphocyte populations, the lectin although not mitogenic, elicits the production of lymphokines from murine splenocytes. Agarose bound Wisteria Floribunda Lectin is prepared from affinity-purified lectin. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x10e7 are used as the solid-phase matrix to which the lectin is covalently bound. The attachment of the lectin to the solid phase is carefully controlled in order to preserve the activity of the lectin as well as to minimize conformational changes of the bound lectin which might result in nonspecific ionic or hydrophobic interactions. The technique developed to couple lectins to agarose provides a very hydrophilic spacer arm between the protein and the matrix. This ensures maximum expression of the carbohydrate binding activity of the lectin. The linkage is very stable over a range of pH values and, unlike cyanogen bromide linkages, proteins are not leached off the gel by Tris or other routinely used buffers. In addition, residual charges generated during cyanogen bromide conjugation which can produce nonspecific binding are not present on the gel following our coupling procedure.
Product Categories/Family for Wisteria floribunda lectin
Similar Products
Product Notes
The Wisteria floribunda (Catalog #AAA656747) is a Lectin produced from Wisteria floribunda seeds and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Wisteria floribunda can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in ELISA. Researchers should empirically determine the suitability of the Wisteria floribunda for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wisteria floribunda, Lectin" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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