Artocarpus integrifolia Lectin

Artocarpus integrifolia (Jacalin, Jackfruit) (Agarose)

Cat. Num. AAA656198

• Applications: ELISA
• Purity: Molecular Biology Grade
• Synonyms: Artocarpus integrifolia; Artocarpus integrifolia (Jacalin; Jackfruit) (Agarose); Artocarpus integrifolia lectin

• Purity/Purification: Molecular Biology Grade
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.5, 0.15M sodium chloride, 0.1mM Ca++, 20mM galactose, 20mM lactose, 0.08% sodium azide. Labeled with Agarose.

• Applicable Applications for Artocarpus integrifolia lectin: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.
• Activity: 2x10e7
• Binding capacity: 4mg asialo-fetuin/ml of gel; 4mg monomeric IgA/ml of gel
• Inhibiting/Eluting Sugar: 800mM galactose or 100mM melibiose 4mg lectin/ml gel
• Preparation and Storage: May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Small intestine: Jacalin (brown, DAB), Hematoxylin counterstain.)

Related Product Information for Artocarpus integrifolia lectin

Jacalin is a lectin composed of four subunits, two of approximately 10,000D and two of 16,000D each. This 50,000D glycoprotein appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl (b-1,3) N-acetylgalactosamine. This structure (the so-called "T-antigen") is the oligosaccharide to which peanut agglutinin binds. However, unlike PNA, Jacalin will bind this structure even in a mono- or disialylated form. This lectin has been used to purify human IgA, since no other human immunoglobulin class binds Jacalin. The specificity of this lectin also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains. Agarose bound Jacalin is prepared from affinity-purified lectin. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x10e7 are used as the solid-phase matrix to which the lectin is covalently bound. The attachment of the lectin to the solid phase is carefully controlled in order to preserve the activity of the lectin as well as to minimize conformational changes of the bound lectin which might result in nonspecific ionic or hydrophobic interactions. acalin is a lectin present in the seeds of the Jackfruit, Artocarpus integrifolia. Recent papers 1,2 reported that Jacalin specifically bound human secretory IgA and that this lectin could be used to sepa- rate human IgA from other serum glycoproteins, including other immunoglobulin classes, and that agarose bound Jacalin could be used to separate IgA1 and IgA2. We have recently affinity purified Jacalin. Our preliminary investigation shows it to have a molecular weight of approximately 60,000 with a rather complex subunit structure. In addition to confirming its affinity toward galactose, we have examined the specificity of this lectin toward several glycoproteins. Using glycoproteins of known carbohydrate structure, we can now provide some information on the structural requirements of the oligosaccharide for binding Jacalin.

Product Categories/Family for Artocarpus integrifolia lectin

Lectins; Lectin Artocarpus integrifolia