Testing Data
(Colon: Dolichos biflorus agglutinin (black, DAB+Ni), using D8085-07A and Alcian blue counterstain.)
Dolichos biflorus Agglutinin Lectin
Dolichos biflorus Agglutinin (DBA, Horse gram) (FITC)
Applications
ELISA
Purity
Molecular Biology Grade
Synonyms
Dolichos biflorus Agglutinin; Dolichos biflorus Agglutinin (DBA; Horse gram) (FITC); Dolichos biflorus Agglutinin lectin
Purity/Purification
Molecular Biology Grade
Form/Format
Supplied as a liquid in 10mM HEPES, 0.15M sodium chloride, pH 7.5, 0.08% sodium azide, 0.1mM Ca++. Labeled with Fluorescein.
Applicable Applications for Dolichos biflorus Agglutinin lectin
ELISA (EL/EIA)
Application Notes
Suitable for use in ELISA.
Emission
515nm
Excitation
495nm
F/P (molar)
2.1
Inhibiting/Eluting Sugar
200mM N-acetylgalactosamine
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Colon: Dolichos biflorus agglutinin (black, DAB+Ni), using D8085-07A and Alcian blue counterstain.)
Testing Data
(Colon: Dolichos biflorus agglutinin (black, DAB+Ni), using D8085-07A and Alcian blue counterstain.)
Related Product Information for Dolichos biflorus Agglutinin lectin
Dolichos biflorus agglutinin is a glycoprotein with a molecular weight of about 120,000 and consists of 4 subunits of approximately equal size. This lectin has an isoelectric point of about pH 5.5 and a carbohydrate specificity toward a-linked N-acetylgalactosamine. DBA is approximately 5000 times more active in agglutinating blood group A1 cells versus A2 cells, and is used to distinguish these blood types in routine serology. It has also been used to establish secretor status in A1 individuals by hemagglutination inhibition techniques and has potential applications in examining tumors which affect the levels of blood group substances. Fluorescein labeled Dolichos Biflorus Agglutinin is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled Dolichos Biflorus Agglutinin has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide.
Product Categories/Family for Dolichos biflorus Agglutinin lectin
Similar Products
Product Notes
The Dolichos biflorus Agglutinin (Catalog #AAA656861) is a Lectin and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Dolichos biflorus Agglutinin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in ELISA. Researchers should empirically determine the suitability of the Dolichos biflorus Agglutinin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dolichos biflorus Agglutinin, Lectin" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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