Rat Vitamin K epoxide reductase complex subunit 1 ELISA Kit | Vkorc1 elisa kit

Rat Vitamin K epoxide reductase complex subunit 1 ELISA Kit

Cat. Num. AAA9425702

• Reactivity: Rat
• Synonyms: Vitamin K epoxide reductase complex subunit 1; Rat Vitamin K epoxide reductase complex subunit 1 ELISA Kit; Vitamin K1 2; 3-epoxide reductase subunit 1; Vkorc1; 1.1.4.1; Vkorc1 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural Rat Superoxide dismutase [Cu-Zn]. No significant cross-reactivity or interference was observed.

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, And Other Biological Fluids
• Assay Type: Quantitative Competitive
• Detection Range: 1.56-100 U/mL.
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Vkorc1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of rat Superoxide dismutase [Cu-Zn] concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide dismutase [Cu-Zn], During the reaction, Superoxide dismutase [Cu-Zn] in the sample or standard competes with a fixed amount of biotin-labeled Superoxide dismutase [Cu-Zn] for sites on a pre-coated Monoclonal antibody specific to Superoxide dismutase [Cu-Zn]. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Superoxide dismutase [Cu-Zn] in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 42627871
• NCBI GeneID: 309004
• NCBI Accession #: NP_976080.1
• NCBI GenBank Nucleotide #: NP_976080.1
• UniProt Accession #: Q6TEK4
• Molecular Weight: 17,783 Da
• NCBI Official Full Name: vitamin K epoxide reductase complex subunit 1
• NCBI Official Synonym Full Names: vitamin K epoxide reductase complex, subunit 1
• NCBI Official Symbol: Vkorc1
• NCBI Protein Information: vitamin K epoxide reductase complex subunit 1
• UniProt Protein Name: Vitamin K epoxide reductase complex subunit 1
• Protein Family: Vitamin K epoxide reductase complex
• UniProt Gene Name: Vkorc1

NCBI Description

Vitamin K is essential for blood clotting but must be enzymatically activated. This enzymatically activated form of vitamin K is a reduced form required for the carboxylation of glutamic acid residues in some blood-clotting proteins. The product of this gene encodes the enzyme that is responsible for reducing vitamin K 2,3-epoxide to the enzymatically activated form. Fatal bleeding can be caused by vitamin K deficiency and by the vitamin K antagonist warfarin, and it is the product of this gene that is sensitive to warfarin. In humans, mutations in this gene can be associated with deficiencies in vitamin-K-dependent clotting factors and, in humans and rats, with warfarin resistance. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in vitamin K metabolism. Catalytic subunit of the vitamin K epoxide reductase (VKOR) complex which reduces inactive vitamin K 2,3-epoxide to active vitamin K. Vitamin K is required for the gamma-carboxylation of various proteins, including clotting factors, and is required for normal blood coagulation, but also for normal bone development.