Rat Superoxide dismutase ELISA Kit | Sod1 elisa kit

Rat Superoxide dismutase [Cu-Zn] ELISA Kit

Cat. Num. AAA9425859

• Gene Names: Sod1; CuZnSOD
• Reactivity: Rat
• Synonyms: Superoxide dismutase; Rat Superoxide dismutase [Cu-Zn] ELISA Kit; Superoxide dismutase [Cu-Zn]; Sod1; 1.15.1.1; Sod1 elisa kit

• Reactivity: Rat
• Specificity: This assay recognizes recombinant and natural rat rMCP-2. No significant cross-reactivity or interference was observed.

• Samples: Serum, Plasma, Tissue Homogenates And Cell Culture Supernates And Other Biological Fluids
• Detection Range: 1.56 ng/ml-100 ng/ml.
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Sod1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of rat rMCP-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to rMCP-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for rMCP-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain rMCP-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of rMCP-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8394328
• NCBI GeneID: 24786
• NCBI Accession #: NP_058746.1
• NCBI GenBank Nucleotide #: NM_017050.1
• UniProt Accession #: P07632
• Molecular Weight: 15,912 Da
• NCBI Official Full Name: superoxide dismutase
• NCBI Official Synonym Full Names: superoxide dismutase 1
• NCBI Official Symbol: Sod1
• NCBI Official Synonym Symbols: CuZnSOD
• NCBI Protein Information: superoxide dismutase [Cu-Zn]
• UniProt Protein Name: Superoxide dismutase [Cu-Zn]
• Protein Family: Superoxide dismutase
• UniProt Gene Name: Sod1

NCBI Description

catalyzes the conversion of superoxide to hydrogen peroxide and molecular oxygen; involved in response to oxidative stress [RGD, Feb 2006]

Uniprot Description

SOD1: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Homodimer; non-disulfide linked. Homodimerization may take place via the ditryptophan cross-link at Trp-33. The pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 interact with RNF19A, whereas wild-type protein does not. The pathogenic variants ALS1 Arg-86 and Ala-94 interact with MARCH5, whereas wild-type protein does not. Belongs to the Cu-Zn superoxide dismutase family.

Protein type: Apoptosis; EC 1.15.1.1; Mitochondrial; Nuclear receptor co-regulator; Oxidoreductase

Chromosomal Location of Human Ortholog: 11q11

Cellular Component: cell soma; cytoplasm; cytoplasmic vesicle; cytosol; dendrite cytoplasm; extracellular matrix; extracellular region; extracellular space; lysosome; mitochondrial intermembrane space; mitochondrion; myelin sheath; neuron projection; nucleoplasm; nucleus; peroxisome; plasma membrane; protein complex; secretory granule

Molecular Function: chaperone binding; copper ion binding; enzyme binding; identical protein binding; protein phosphatase 2B binding; Rac GTPase binding; superoxide dismutase activity; zinc ion binding

Biological Process: activation of MAPK activity; aging; anterograde axon cargo transport; auditory receptor cell stereocilium organization and biogenesis; cell aging; cellular iron ion homeostasis; DNA fragmentation during apoptosis; double-strand break repair; embryo implantation; glutathione metabolic process; heart contraction; hydrogen peroxide biosynthetic process; locomotory behavior; muscle maintenance; myelin maintenance in the peripheral nervous system; myeloid cell homeostasis; negative regulation of apoptosis; negative regulation of cholesterol biosynthetic process; negative regulation of neuron apoptosis; neurofilament cytoskeleton organization and biogenesis; ovarian follicle development; positive regulation of catalytic activity; positive regulation of cytokine production; positive regulation of superoxide release; regulation of blood pressure; regulation of GTPase activity; regulation of mitochondrial membrane potential; regulation of multicellular organism growth; regulation of protein kinase activity; relaxation of vascular smooth muscle; removal of superoxide radicals; response to amphetamine; response to antibiotic; response to axon injury; response to copper ion; response to drug; response to ethanol; response to heat; response to hydrogen peroxide; response to nutrient levels; response to organic substance; response to oxidative stress; response to reactive oxygen species; response to superoxide; retinal homeostasis; retrograde axon cargo transport; sensory perception of sound; spermatogenesis; superoxide metabolic process; superoxide release; transmission of nerve impulse