Typical Testing Data/Standard Curve (for reference only)
Human Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1) ELISA Kit | VKORC1 elisa kit
Human Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to VKORC1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to VKORC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VKORC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VKORC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the catalytic subunit of the vitamin K epoxide reductase complex, which is responsible for the reduction of inactive vitamin K 2,3-epoxide to active vitamin K in the endoplasmic reticulum membrane. Vitamin K is a required co-factor for carboxylation of glutamic acid residues by vitamin K-dependent gamma-carboxylase in blood-clotting enzymes. Allelic variation in this gene is associated with vitamin k-dependent clotting factors combined deficiency of 2, and increased resistance or sensitivity to warfarin, an inhibitor of vitamin K epoxide reductase. Pseudogenes of this gene are located on chromosomes 1 and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
Uniprot Description
VKORC1: Involved in vitamin K metabolism. Catalytic subunit of the vitamin K epoxide reductase (VKOR) complex which reduces inactive vitamin K 2,3-epoxide to active vitamin K. Defects in VKORC1 are a cause of combined deficiency of vitamin K-dependent clotting factors type 2 (VKCFD2). VKCFD leads to a bleeding tendency that is usually reversed by oral administration of vitamin K. Defects in VKORC1 are a cause of coumarin resistance (CMRES). A condition characterized by partial or complete resistance to warfarin or other 4-hydroxycoumarin derivatives. These drugs are used as anti-coagulants for the prevention of thromboembolic diseases in subjects with deep vein thrombosis, atrial fibrillation, or mechanical heart valve replacement. Belongs to the VKOR family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Oxidoreductase; EC 1.1.4.1; Membrane protein, integral; Membrane protein, multi-pass; Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: endoplasmic reticulum membrane; integral to membrane
Molecular Function: vitamin-K-epoxide reductase (warfarin-sensitive) activity; quinone binding
Biological Process: cellular protein metabolic process; vitamin K metabolic process; blood coagulation; post-translational protein modification; drug metabolic process; peptidyl-glutamic acid carboxylation
Disease: Coumarin Resistance; Vitamin K-dependent Clotting Factors, Combined Deficiency Of, 2
Research Articles on VKORC1
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Product Notes
The Human VKORC1 vkorc1 (Catalog #AAA2025112) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2025112 ELISA Kit recognizes Human VKORC1. It is sometimes possible for the material contained within the vial of "Vitamin K Epoxide Reductase Complex Subunit 1 (VKORC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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