Rabbit anti-Human, Mouse Insulin Receptor R Polyclonal Antibody | anti-INSRR antibody

Insulin Receptor R, NT (Insulin Receptor-related Protein, Insulin Receptor-related Receptor, IR-related Receptor, INSRR, IRR) (FITC)

Cat. Num. AAA6482009

• Gene Names: INSRR; IRR
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Insulin Receptor R; Polyclonal Antibody; NT (Insulin Receptor-related Protein; Insulin Receptor-related Receptor; IR-related Receptor; INSRR; IRR) (FITC); Insulin Receptor-related Protein alpha Chain; Insulin Receptor-related Protein beta Chain; EC=2.7.10.1; anti-INSRR antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human INSRR. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-INSRR antibody: FLISA, Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 16-47 in the N-terminal region of human INSRR, UniProt Accession #P14616.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of I7661-26R in mouse brain lysate. INSRR (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with I7661-26R, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.)

Related Product Information for anti-INSRR antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Product Categories/Family for anti-INSRR antibody

Antibodies; Receptors; Tyrosine Kinase

References

1. Shier, P., et al., J. Biol. Chem. 264(25):14605-14608 (1989). 2. Whitmore, T.E., et al., Cytogenet. Cell Genet. 87(1-2), 93-94 (1999). 3. Hanze, J., et al., Horm. Metab. Res. 31(2-3), 77-79 (1999). 4. Shier, P., et al., Cytogenet. Cell Genet. 54(1-2), 80-81 (1990). 5. Elmlinger, M.W., et al., Regul. Pept. 84(1-3), 37-42 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 31657140
• NCBI GeneID: 3645
• NCBI Accession #: NP_055030.1
• NCBI GenBank Nucleotide #: NM_014215.2
• UniProt Accession #: P14616; O60724; Q5VZS3
• Molecular Weight: 143,720 Da
• NCBI Official Full Name: insulin receptor-related protein
• NCBI Official Synonym Full Names: insulin receptor-related receptor
• NCBI Official Symbol: INSRR
• NCBI Official Synonym Symbols: IRR
• NCBI Protein Information: insulin receptor-related protein; IR-related receptor
• UniProt Protein Name: Insulin receptor-related protein
• Protein Family: Insulin receptor-related protein
• UniProt Gene Name: INSRR
• UniProt Synonym Gene Names: IRR; IRR
• UniProt Entry Name: INSRR_HUMAN

Uniprot Description

IRR: Receptor with tyrosine-protein kinase activity. Functions as a pH sensing receptor which is activated by increased extracellular pH. Activates an intracellular signaling pathway that involves IRS1 and AKT1/PKB. Probable tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chains carry the kinase domain. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Protein type: Protein kinase, TK; Membrane protein, integral; Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; InsR family

Chromosomal Location of Human Ortholog: 1q21-q23

Cellular Component: integral to plasma membrane; receptor complex

Molecular Function: insulin receptor substrate binding; phosphoinositide 3-kinase binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; male sex determination; actin cytoskeleton reorganization; transmembrane receptor protein tyrosine kinase signaling pathway