USP5 / ISOT recombinant protein

Recombinant Human USP5 / ISOT Protein (His tag)

Cat. Num. AAA2545453

• Gene Names: USP5; ISOT
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: USP5 / ISOT; Recombinant Human USP5 / ISOT Protein (His tag); ISOT; USP5 / ISOT recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.4
• Sequence Length: 858

• Application Notes: The recombinant human USP5 consists of 846 amino acids and predicts a molecular mass of 94.7 kDa. It migrates as an approximately 100 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met 1
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for USP5 / ISOT recombinant protein

Background: Ubiquitin carboxyl-terminal hydrolase 5, also known as Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thiolesterase 5, Ubiquitin-specific-processing protease 5, ISOT and USP5, is a member of the peptidase C19 family. USP5 contains 2 UBA domains and one UBP-type zinc finger. The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition. The UBP-type zinc finger domain crystallizes as a dimer linked by a disulfide bond between the Cys-195 residues of both molecules, but there is no evidence that the full-length USP5 exists as a dimer. USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. USP5 is involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. It binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Description: A DNA sequence encoding the human USP5 isoform short (P45974-2) (Met 1-Ser 835) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 148727331
• NCBI GeneID: 8078
• NCBI Accession #: NP_001092006.1
• NCBI GenBank Nucleotide #: NM_001098536.1
• UniProt Accession #: P45974; Q96J22; D3DUS7; D3DUS8
• Molecular Weight: 93,308 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 5 isoform 1
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 5
• NCBI Official Symbol: USP5
• NCBI Official Synonym Symbols: ISOT
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 5
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 5
• UniProt Gene Name: USP5
• UniProt Synonym Gene Names: ISOT

NCBI Description

Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010]

Uniprot Description

USP5: Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys- 63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. Belongs to the peptidase C19 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Protease; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: lysosome

Molecular Function: cysteine-type endopeptidase activity; protein binding; ubiquitin binding; ubiquitin-specific protease activity

Biological Process: positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination