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Western Blot (WB) (Western BlotPositive WB detected in: Jurkat whole cell lysate, K562 whole cell lysateAll lanes: LDB1 antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47, 37, 43 KDaObserved band size: 47 KDa)

Rabbit anti-Human LDB1 Polyclonal Antibody | anti-LDB1 antibody

LDB1 Antibody

Gene Names
LDB1; NLI; CLIM2; LDB-1; CLIM-2
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
LDB1; Polyclonal Antibody; LDB1 Antibody; LIM domain-binding protein 1; LDB-1; Carboxyl-terminal LIM domain-binding protein 2; CLIM-2; LIM domain-binding factor CLIM2; hLdb1; Nuclear LIM interactor; CLIM2; anti-LDB1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
411
Applicable Applications for anti-LDB1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:200
Immunogen
Recombinant Human LIM domain-binding protein 1 protein (50-375AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Jurkat whole cell lysate, K562 whole cell lysateAll lanes: LDB1 antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47, 37, 43 KDaObserved band size: 47 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Jurkat whole cell lysate, K562 whole cell lysateAll lanes: LDB1 antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 47, 37, 43 KDaObserved band size: 47 KDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7103981 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver cancer using MBS7103981 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of Hela cells using MBS7103981 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunohistochemistry (IHC) (Immunofluorescent analysis of Hela cells using MBS7103981 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-LDB1 antibody
Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. Plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state (By similarity).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,809 Da
NCBI Official Full Name
LIM domain-binding protein 1 isoform 1
NCBI Official Synonym Full Names
LIM domain binding 1
NCBI Official Symbol
LDB1
NCBI Official Synonym Symbols
NLI; CLIM2; LDB-1; CLIM-2
NCBI Protein Information
LIM domain-binding protein 1
UniProt Protein Name
LIM domain-binding protein 1
UniProt Gene Name
LDB1
UniProt Synonym Gene Names
CLIM2; LDB-1; CLIM-2; hLdb1

Uniprot Description

Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. Plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state ().

Research Articles on LDB1

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Product Notes

The LDB1 ldb1 (Catalog #AAA7103981) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LDB1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LDB1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the LDB1 ldb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LDB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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