Immunohistochemistry (IHC)
(Immunochemical staining of human USP5 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human USP5 Polyclonal Antibody | anti-USP5 antibody
Anti-USP5 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
USP5; Polyclonal Antibody; Anti-USP5 Antibody; Rabbit Polyclonal; USP5 Antibody; Rabbit PAb; Antigen Affinity Purified; ubiquitin specific peptidase 5 (isopeptidase T); Anti-ISOT Antibody; anti-USP5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human USP5
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-USP5 antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:50-1:200
Immunogen
E Coli-derived Human USP5 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human USP5 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human USP5 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human USP5 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-USP5 antibody
Ubiquitin carboxyl-terminal hydrolase 5, also known as Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thiolesterase 5, Ubiquitin-specific-processing protease 5, ISOT and USP5, is a member of the peptidase C19 family. USP5 contains 2 UBA domains and one UBP-type zinc finger. The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition. The UBP-type zinc finger domain crystallizes as a dimer linked by a disulfide bond between the Cys-195 residues of both molecules, but there is no evidence that the full-length USP5 exists as a dimer. USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. USP5 is involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. It binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
References
Reyes-Turcu F.E., et al., 2006, Cell 124:1197-1208. Reyes-Turcu F.E., et al., 2008, J. Biol. Chem. 283:19581-19592. Dayal S., et al., 2009, J. Biol. Chem. 284:5030-5041. Burkard T.R., et al., 2011, BMC Syst. Biol. 5:17-17.
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Product Notes
The USP5 (Catalog #AAA8109454) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-USP5 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's USP5 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the USP5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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