Mouse IAPP ELISA Kit | IAPP elisa kit

Mouse IAPP (Islet Amyloid Polypeptide) ELISA Kit

Cat. Num. AAA2515651

• Gene Names: IAPP; DAP; IAP
• Reactivity: Mouse
• Synonyms: IAPP; Mouse IAPP (Islet Amyloid Polypeptide) ELISA Kit; IAPP elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant Mouse IAPP. No significant cross-reactivity or interference between Mouse IAPP and analogues was observed.
• Sequence Length: 89

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Competitive
• Detection Range: 62.5-4000pg/mL
• Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IAPP elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse IAPP concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with IAPP. During the reaction, IAPP in the sample or standard competes with a fixed amount of IAPP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to IAPP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IAPP in the samples is then determined by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557655
• NCBI GeneID: 3375
• NCBI Accession #: NP_000406.1
• NCBI GenBank Nucleotide #: NM_000415.2
• Molecular Weight: 9,806 Da
• NCBI Official Full Name: islet amyloid polypeptide
• NCBI Official Synonym Full Names: islet amyloid polypeptide
• NCBI Official Symbol: IAPP
• NCBI Official Synonym Symbols: DAP; IAP
• NCBI Protein Information: islet amyloid polypeptide; Islet amyloid polypeptide (diabetes-associated peptide; amylin); insulinoma amyloid peptide
• UniProt Protein Name: Islet amyloid polypeptide
• Protein Family: Islet amyloid polypeptide
• UniProt Gene Name: IAPP
• UniProt Synonym Gene Names: DAP
• UniProt Entry Name: IAPP_HUMAN

NCBI Description

The protein encoded by this gene is commonly found in pancreatic islets of patients suffering diabetes mellitus type II, or harboring an insulinoma. Studies suggest that this protein, like the related beta-amyloid (Abeta) associated with Alzheimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the development of type II diabetes. This protein also exhibits an bactericidal, antimicrobial activity. [provided by RefSeq, Sep 2014]

Uniprot Description

IAPP: Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism. Belongs to the calcitonin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: extracellular space; cell soma; extracellular region

Molecular Function: identical protein binding; hormone activity; receptor binding

Biological Process: negative regulation of cell differentiation; cell-cell signaling; apoptosis; eating behavior; signal transduction; sensory perception of pain; negative regulation of bone resorption; endocrine pancreas development