NEDD8 activating enzyme E1 Recombinant Protein | NEDD8 recombinant protein

NEDD8 activating enzyme E1 (human), (recombinant) (His-tag)

Cat. Num. AAA567195

• Gene Names: UBA3; NAE2; UBE1C; hUBA3
• Purity: >=95% (SDS-PAGE); Copurified in His-tagged form with Uba3; copurified with APP-BP1
• Synonyms: NEDD8 activating enzyme E1; NEDD8 activating enzyme E1 (human); (recombinant) (His-tag); NEDD8 recombinant protein

• Host: E Coli
• Purity/Purification: >=95% (SDS-PAGE); Copurified in His-tagged form with Uba3; copurified with APP-BP1
• Form/Format: Liquid. In 25mM HEPES, pH7.5, containing 150mM NaCl, 1mM DTT

• Preparation and Storage: For long term storage, store at -80 degree C; Shipping: Shipped on Dry Ice

Related Product Information for NEDD8 recombinant protein

Conjugation of mature NEDD8 to specific lysine residues on target proteins via isopeptide bonds allows NEDD8 to play a critical regulatory role in cell proliferation and development, a process closely associated to the ubiquitin-proteasome pathway. An increasing number of substrates for NEDDylation are being identified including the tumor suppressors p53 and VHL and members of the well-characterized cullin ubiquitin E3 ligase family, underlining the vital role NEDD8 modification plays in cellular processes.

Product Categories/Family for NEDD8 recombinant protein

Cellular Analysis; Stem Cells; Proteostasis; Protein Synthesis & Folding; Posttranslational Modifications; Ubiquitin & UBL Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 83305811
• NCBI GeneID: 9039
• UniProt Accession #: Q8TBC4; O76088; Q9NTU3; A6NLB5; A8K027
• Molecular Weight: 463
• NCBI Official Full Name: NEDD8-activating enzyme E1 catalytic subunit
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 3
• NCBI Official Symbol: UBA3
• NCBI Official Synonym Symbols: NAE2; UBE1C; hUBA3
• NCBI Protein Information: NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1C; Nedd8-activating enzyme hUba3; ubiquitin-activating enzyme 3; NEDD8-activating enzyme E1 subunit 2; ubiquitin-like modifier-activating enzyme 3; UBA3, ubiquitin-activating enzyme E
• UniProt Protein Name: NEDD8-activating enzyme E1 catalytic subunit
• Protein Family: NEDD8
• UniProt Gene Name: UBA3
• UniProt Synonym Gene Names: UBE1C; Ubiquitin-activating enzyme 3
• UniProt Entry Name: UBA3_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

UBA3: Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. Belongs to the ubiquitin-activating E1 family. UBA3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; EC 6.3.2.19; Ubiquitin ligase; Ligase; Ubiquitin conjugating system; Cell cycle regulation; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: nucleus; cytosol

Molecular Function: NEDD8 activating enzyme activity; protein binding; acid-amino acid ligase activity; protein heterodimerization activity; ATP binding

Biological Process: protein neddylation; regulation of cell cycle; protein modification process; endomitotic cell cycle; proteolysis