NAE1/UBA3 Complex Recombinant Protein | NAE1/UBA3 recombinant protein

NAE1/UBA3 Complex, Active

Cat. Num. AAA517739

• Gene Names: UBA3; NAE2; UBE1C; hUBA3
• Synonyms: NAE1/UBA3 Complex; Active; NAE1 -APPBP1 UBA3 -UBE1C; UBA3; hUBA3; NAE1/UBA3 recombinant protein

• Host: Sf9 insect cells.
• Form/Format: Recombinant protein complex is a combination of the following 2 buffer systems, adjusted to optimize the NAE1 (phosphate buffer system) and UBA3 (tris buffer system) proportions:; 50mM sodium phosphate, pH7.5, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25

• Tag: His/GST
• Species: Human
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Activity

(Specific Activity: The specific activity of NAE1/UBA3 was determined to be 6.4 nmol/min/mg as per activity assay protocol.)

SDS-Page

(Purity: The purity of NAE1/UBA3 was determined to be >70% by densitometry. NAE1 approx. MW 65 kDa. UBA3 approx. MW 73 kDa.)

Related Product Information for NAE1/UBA3 recombinant protein

Recombinant human NAE1 (2-end) and full-length human UBA3 were expressed by baculovirus in Sf9 insect cells. NAE1 used an N-terminal His tag, and the gene accession number is NM_003905. UBA3 used an N-terminal GST tag, and the gene accession number is BC022853.
NAE1/UBA3 is a heterodimeric E1 (Ub-activating)-like complex that activates NEDD8. In the presence of ATP, NAE1/UBA3 adenylates and activates NEDD8 yielding NEDD8-UBA3 thioester and free AMP. Activated NEDD8 is then transferred to UBE2M or UBE2F through transthiolyation reaction. Finally, substrate specific-E3 ligase, such as RBX1/2 or DCN1, transfers NEDD8 from UBE2M or UBE2F to a lysine residue in its target protein (1,2). NEDD8 pathway plays an important role in cell cycle progression and survival (3).

Product Categories/Family for NAE1/UBA3 recombinant protein

Ubiquitin Enzymes; Cell Cycle

References

1. Zhou, L. et al: Neddylation: a novel modulator of the tumor microenvironment. Mol Cancer. 18: 77, 2019. 2. Gong, L. et al: Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway. J Biol Chem. 23 (274):12036-42, 1999. 3. Soucy, T.A. et al: The NEDD8 conjugation pathway and its relevance in cancer biology and therapy. Genes Cancer. 1: 708-716, 2010.

NCBI and Uniprot Product Information

• NCBI GI #: 18605782
• NCBI GeneID: 9039
• NCBI Accession #: BC022853
• NCBI GenBank Nucleotide #: NM_003905
• Molecular Weight: 50,072 Da
• NCBI Official Full Name: Homo sapiens ubiquitin-like modifier activating enzyme 3, mRNA
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 3
• NCBI Official Symbol: UBA3
• NCBI Official Synonym Symbols: NAE2; UBE1C; hUBA3
• NCBI Protein Information: NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1 subunit 2; NEDD8-activating enzyme E1C; Nedd8-activating enzyme hUba3; UBA3, ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme 3; ubiquitin-activating enzyme E1C (U

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]