Rabbit anti-Human UBA3 Polyclonal Antibody | anti-UBA3 antibody

UBA3 Polyclonal Antibody

Cat. Num. AAA9432427

• Gene Names: UBA3; NAE2; UBE1C; hUBA3
• Reactivity: Human
• Applications: Immunofluorescence
• Purity: Affinity purification
• Synonyms: UBA3; Polyclonal Antibody; UBA3 Polyclonal Antibody; NAE2; UBE1C; hUBA3; ubiquitin like modifier activating enzyme 3; anti-UBA3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 50% glycerol, pH7.4

• Applicable Applications for anti-UBA3 antibody: Immunofluorescence (IF)
• Application Notes: IF: 1:50-1:200
• Immunogen: Recombinant fusion protein of human UBA3 (NP_003959.3).
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells using UBA3 antibody.)

Related Product Information for anti-UBA3 antibody

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Product Categories/Family for anti-UBA3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 38045942
• NCBI GeneID: 9039
• NCBI Accession #: NP_003959.3
• NCBI GenBank Nucleotide #: NM_003968.3
• UniProt Accession #: Q8TBC4; O76088; Q9NTU3; A6NLB5; A8K027
• Molecular Weight: 50,072 Da
• NCBI Official Full Name: NEDD8-activating enzyme E1 catalytic subunit isoform 1
• NCBI Official Synonym Full Names: ubiquitin-like modifier activating enzyme 3
• NCBI Official Symbol: UBA3
• NCBI Official Synonym Symbols: NAE2; UBE1C; hUBA3
• NCBI Protein Information: NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1 subunit 2; NEDD8-activating enzyme E1C; Nedd8-activating enzyme hUba3; UBA3, ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme 3; ubiquitin-activating enzyme E1C (U
• UniProt Protein Name: NEDD8-activating enzyme E1 catalytic subunit
• Protein Family: Uba3-binding protein
• UniProt Gene Name: UBA3
• UniProt Synonym Gene Names: UBE1C; Ubiquitin-activating enzyme 3
• UniProt Entry Name: UBA3_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

UBA3: Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. Belongs to the ubiquitin-activating E1 family. UBA3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; EC 6.3.2.19; Ubiquitin ligase; Ligase; Ubiquitin conjugating system; Cell cycle regulation; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: nucleus; cytosol

Molecular Function: NEDD8 activating enzyme activity; protein binding; acid-amino acid ligase activity; protein heterodimerization activity; ATP binding

Biological Process: protein neddylation; regulation of cell cycle; protein modification process; endomitotic cell cycle; proteolysis