Mouse anti-Human WTAP Monoclonal Antibody | anti-WTAP antibody

Anti-WTAP Antibody (clone 1B11)

Cat. Num. AAA2401137

• Gene Names: WTAP; Mum2
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified from ascites by Protein A
• Synonyms: WTAP; Monoclonal Antibody; Anti-WTAP Antibody (clone 1B11); Rabbit Polyclonal (IgG) to Human WTAP; HFL(2)D; KIAA0105; PNAS-132; WT1-associated protein; Female-lethal(2)D homolog; anti-WTAP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, k
• Clone Number: 1B11
• Specificity: Human WTAP
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4.
• Sequence Length: 396

• Applicable Applications for anti-WTAP antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3-10 ug/ml
• Immunogen: WTAP (NP_690596, 70 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C or lower.; Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

ELISA (EIA)

(Detection limit for recombinant GST tagged WTAP is 0.1 ng/ml as a capture antibody.)

Related Product Information for anti-WTAP antibody

Regulatory subunit of the WMM N6-methyltransferase complex, amultiprotein complex that mediates N6-methyladenosine (m6A) methylationof some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing and processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as amRNA splicing regulator. Regulates G2/M cell-cycle transition by bindingto the 3' UTR of CCNA2, which enhances its stability.

NCBI and Uniprot Product Information

• NCBI GI #: 21361159
• NCBI GeneID: 9589
• NCBI Accession #: NP_004897.2
• NCBI GenBank Nucleotide #: NM_004906.4
• UniProt Accession #: Q15007; Q5TCL8; Q5TCL9; Q96T28; Q9BYJ7; Q9H4E2
• Molecular Weight: 17,801 Da
• NCBI Official Full Name: pre-mRNA-splicing regulator WTAP isoform 1
• NCBI Official Synonym Full Names: WT1 associated protein
• NCBI Official Symbol: WTAP
• NCBI Official Synonym Symbols: Mum2
• NCBI Protein Information: pre-mRNA-splicing regulator WTAP
• UniProt Protein Name: Pre-mRNA-splicing regulator WTAP
• Protein Family: Pre-mRNA-splicing regulator
• UniProt Gene Name: WTAP
• UniProt Synonym Gene Names: KIAA0105; hFL(2)D

NCBI Description

The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).