TXNL4B recombinant protein

TXNL4B Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121439

• Gene Names: TXNL4B; DLP; Dim2
• Purity: >92% as determined by SDS-PAGE
• Synonyms: TXNL4B; TXNL4B Protein; Human; Recombinant (His Tag); Human Dim2/TXNL4B Protein (His Tag); Dim2 Protein; DLP Protein; thioredoxin-like 4B; TXNL4B recombinant protein

• Host: E Coli
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 20% glycerol, 0.1% Tween20, 50mM Arg, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ile149

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human TXNL4B (Q9NX01)(Met 1-Ile 149) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TXNL4B recombinant protein

Background: Dim2, also known as TXNL4B, is a member of the DIM1 family. The Dim protein family is composed of two classes, Dim1and Dim2, which share a common thioredoxin-like fold. They were originally identified for their role in cell cycle progression and have been found to interact with Prp6, an essential component of the spliceosome, which forms the bridge of U4/U6.U5-tri-snRNP. Despite their biological and structural similarities, Dim1 and Dim2 proteins differ in many aspects. Dim1 bears distinctive structural motifs responsible for its interaction with other spliceosome components. Dim2 forms homodimers and contains specific domains required for its interactions with partners. This originality suggests that although both proteins are involved in pre-mRNA splicing, they are likely to be involved in different biological pathways. Dim2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 185 amino acids and having a molecular mass of 21.1kDa. It is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Dim2 has a vital role in pro-mRNA splicing. Dim2 is required in cell cycle progression for S/G2 transition and interacts with PRPF6 subunit of the spliceosome.

References

Sun X, et al. (2004) DLP, a novel Dim1 family protein implicated in pre-mRNA splicing and cell cycle progression. J Biol Chem. 279(31):32839-47.Maruyama K, et al. (1994) Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene. 138(1-2):171-4.Suzuki Y, et al. (1997) Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library. Gene. 200(1-2):149-56.

NCBI and Uniprot Product Information

• NCBI GI #: 214832254
• NCBI GeneID: 54957
• NCBI Accession #: NP_001135789.1
• NCBI GenBank Nucleotide #: NM_001142317.1
• UniProt Accession #: Q9NX01; D3DWS6
• Molecular Weight: 17,015 Da
• NCBI Official Full Name: thioredoxin-like protein 4B
• NCBI Official Synonym Full Names: thioredoxin-like 4B
• NCBI Official Symbol: TXNL4B
• NCBI Official Synonym Symbols: DLP; Dim2
• NCBI Protein Information: thioredoxin-like protein 4B; Dim1-like protein
• UniProt Protein Name: Thioredoxin-like protein 4B
• Protein Family: Thioredoxin-like protein
• UniProt Gene Name: TXNL4B
• UniProt Synonym Gene Names: DIM2; DLP
• UniProt Entry Name: TXN4B_HUMAN

Uniprot Description

TXNL4B: Essential role in pre-mRNA splicing. Required in cell cycle progression for S/G(2) transition. Belongs to the DIM1 family.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: spliceosome; U4/U6 x U5 tri-snRNP complex; snRNP U5

Biological Process: mitosis; spliceosome assembly