SDS-Page
SPF30 Recombinant Protein | SMNDC1 recombinant protein
SPF30 Protein, Human, Recombinant (His Tag)
Gene Names
SMNDC1; SMNR; SPF30; TDRD16C
Purity
>85% as determined by SDS-PAGE
Synonyms
SPF30; SPF30 Protein; Human; Recombinant (His Tag); Human SMNDC1/ SPF30 Protein (His Tag); SMNR Protein; TDRD16C Protein; survival motor neuron domain containing 1; SMNDC1 recombinant protein
Host
E Coli
Purity/Purification
>85% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, 20% Glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Gln238
Species
Human
Predicted N Terminal
His
Tag
N-His
Protein Construction
A DNA sequence encoding the mature form of human SMNDC1 (O75940)(Met1-Gln238) was expressed with a polyhistide tag at the N-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for SMNDC1 recombinant protein
Background: SMNDC1 gene is a paralog of SMN1 gene, which encodes the survival motor neuron protein, mutations in which are cause of autosomal recessive proximal spinal muscular atrophy. SMNDC1 is a nuclear protein that has been identified as a constituent of the spliceosome complex. SMNDC1 gene is differentially expressed, with abundant levels in skeletal muscle, and may share similar cellular function as the SMN1 gene. SMNDC1 is necessary for spliceosome assembly. Its overexpression causes apoptosis.
References
Rappsilber J, et al. (2001) SPF30 is an essential human splicing factor required for assembly of the U4/U5/U6 tri-small nuclear ribonucleoprotein into the spliceosome. J Biol Chem. 276(33): 31142-50. Talbot K, et al. (1999) Characterization of a gene encoding survival motor neuron (SMN)-related protein, a constituent of the spliceosome complex. Hum Mol Genet. 7(13): 2149-56. Neubauer G, et al. (1998) Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex. Nat Genet. 20(1):46-50.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
238
NCBI Official Full Name
Survival of motor neuron-related-splicing factor 30
NCBI Official Synonym Full Names
survival motor neuron domain containing 1
NCBI Official Symbol
SMNDC1
NCBI Official Synonym Symbols
SMNR; SPF30; TDRD16C
NCBI Protein Information
survival of motor neuron-related-splicing factor 30; SMN-related protein; tudor domain containing 16C; 30 kDa splicing factor SMNrp; survival motor neuron domain-containing protein 1; splicing factor 30, survival of motor neuron-related
UniProt Protein Name
Survival of motor neuron-related-splicing factor 30
Protein Family
UniProt Gene Name
SMNDC1
UniProt Synonym Gene Names
SMNR; SPF30
UniProt Entry Name
SPF30_HUMAN
NCBI Description
This gene is a paralog of SMN1 gene, which encodes the survival motor neuron protein, mutations in which are cause of autosomal recessive proximal spinal muscular atrophy. The protein encoded by this gene is a nuclear protein that has been identified as a constituent of the spliceosome complex. This gene is differentially expressed, with abundant levels in skeletal muscle, and may share similar cellular function as the SMN1 gene. [provided by RefSeq, Jul 2008]
Uniprot Description
SPF30: Necessary for spliceosome assembly. Overexpression causes apoptosis. Belongs to the SMN family.
Protein type: Spliceosome; RNA processing; RNA splicing; RNA-binding
Chromosomal Location of Human Ortholog: 10q23
Cellular Component: nucleoplasm; spliceosome; Cajal body; intermediate filament cytoskeleton; cytoplasm; nuclear speck; nucleus
Molecular Function: protein binding
Biological Process: RNA splicing, via transesterification reactions; apoptosis; RNA splicing; spliceosome assembly; mRNA processing
Research Articles on SMNDC1
Similar Products
Product Notes
The SMNDC1 smndc1 (Catalog #AAA8121663) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Gln23 8. It is sometimes possible for the material contained within the vial of "SPF30, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
