Canine Thromboxane A2 ELISA Kit | TX-A2 elisa kit

Canine Thromboxane A2 ELISA Kit

Cat. Num. AAA738044

• Gene Names: TBXA2R; TXA2-R; BDPLT13
• Reactivity: Canine
• Synonyms: Thromboxane A2; Canine Thromboxane A2 ELISA Kit; TX-A2 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This TXA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine TXA2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TX-A2 elisa kit

Principle of the Assay: TXA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TXA2 antibody and an TXA2-HRP conjugate. The assay sample and buffer are incubated together with TXA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TXA2 concentration since TXA2 from samples and TXA2-HRP conjugate compete for the anti-TXA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by TXA2 from the sample, fewer sites are left to bind TXA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TXA2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TX-A2 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 260593681
• NCBI GeneID: 6915
• NCBI Accession #: NP_963998.2
• NCBI GenBank Nucleotide #: NM_201636.2
• Molecular Weight: 44,205 Da
• NCBI Official Full Name: thromboxane A2 receptor isoform beta
• NCBI Official Synonym Full Names: thromboxane A2 receptor
• NCBI Official Symbol: TBXA2R
• NCBI Official Synonym Symbols: TXA2-R; BDPLT13
• NCBI Protein Information: thromboxane A2 receptor; prostanoid TP receptor
• UniProt Protein Name: Thromboxane A2 receptor
• Protein Family: Thromboxane A2 receptor
• UniProt Gene Name: TBXA2R
• UniProt Synonym Gene Names: TXA2-R
• UniProt Entry Name: TA2R_HUMAN

NCBI Description

This gene encodes a member of the G protein-coupled receptor family. The protein interacts with thromboxane A2 to induce platelet aggregation and regulate hemostasis. A mutation in this gene results in a bleeding disorder. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

TXA2-R: Receptor for thromboxane A2 (TXA2), a potent stimulator of platelet aggregation. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. In the kidney, the binding of TXA2 to glomerular TP receptors causes intense vasoconstriction. Activates phospholipase C. Isoform 1 activates adenylyl cyclase. Isoform 2 inhibits adenylyl cyclase. Defects in TBXA2R are the cause of susceptibility to bleeding disorder platelet-type 13 (BDPLT13). BDPLT13 is a disorder characterized by reduced platelet aggregation and a tendency to mild mucocutaneous bleeding. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: integral to plasma membrane; plasma membrane; acrosome

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; thromboxane A2 receptor activity

Biological Process: second-messenger-mediated signaling; response to drug; platelet activation; response to lipopolysaccharide; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; positive regulation of angiogenesis; positive regulation of vasoconstriction; positive regulation of smooth muscle contraction; inflammatory response; blood coagulation; positive regulation of blood pressure; response to nutrient; positive regulation of GTPase activity