Related Product Information for TX A2 elisa kit
Principle of the assay: TXA2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TXA2. Standards or samples are then added to the microtiter plate wells and TXA2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TXA2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TXA2 are added to each well to "sandwich" the TXA2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TXA2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TXA2 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for TX A2 elisa kit
NCBI Description
This gene encodes a member of the G protein-coupled receptor family. The protein interacts with thromboxane A2 to induce platelet aggregation and regulate hemostasis. A mutation in this gene results in a bleeding disorder. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Uniprot Description
TXA2-R: Receptor for thromboxane A2 (TXA2), a potent stimulator of platelet aggregation. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. In the kidney, the binding of TXA2 to glomerular TP receptors causes intense vasoconstriction. Activates phospholipase C. Isoform 1 activates adenylyl cyclase. Isoform 2 inhibits adenylyl cyclase. Defects in TBXA2R are the cause of susceptibility to bleeding disorder platelet-type 13 (BDPLT13). BDPLT13 is a disorder characterized by reduced platelet aggregation and a tendency to mild mucocutaneous bleeding. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: integral to plasma membrane; acrosome; plasma membrane
Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; thromboxane A2 receptor activity
Biological Process: response to drug; second-messenger-mediated signaling; platelet activation; response to lipopolysaccharide; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; positive regulation of angiogenesis; positive regulation of vasoconstriction; positive regulation of smooth muscle contraction; inflammatory response; blood coagulation; positive regulation of blood pressure; response to nutrient; positive regulation of GTPase activity
Product Notes
The Human TX A2 tbxa2r (Catalog #
AAA724222) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA724222 ELISA Kit recognizes Human TX A2.
It is sometimes possible for the material contained within the vial of
"Thromboxane A2, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
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