Porcine Thromboxane A2 (TX-A2) ELISA Kit | TX-A2 elisa kit

Porcine Thromboxane A2 (TX-A2) ELISA Kit

Cat. Num. AAA260881

• Gene Names: TBXA2R; TXA2-R; BDPLT13
• Reactivity: Porcine
• Synonyms: Thromboxane A2 (TX-A2); Porcine Thromboxane A2 (TX-A2) ELISA Kit; TX-A2 elisa kit

• Reactivity: Porcine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 407

• Samples: Serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 1000 pg/ml-15.6pg/ml
• Sensitivity: Up to 5 pg/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Related Product Information for TX-A2 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine TX-A2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 260593681
• NCBI GeneID: 6915
• NCBI Accession #: NP_963998.2
• NCBI GenBank Nucleotide #: NM_201636.2
• Molecular Weight: 44,205 Da
• NCBI Official Full Name: thromboxane A2 receptor isoform beta
• NCBI Official Synonym Full Names: thromboxane A2 receptor
• NCBI Official Symbol: TBXA2R
• NCBI Official Synonym Symbols: TXA2-R; BDPLT13
• NCBI Protein Information: thromboxane A2 receptor; prostanoid TP receptor
• UniProt Protein Name: Thromboxane A2 receptor
• UniProt Gene Name: TBXA2R
• UniProt Synonym Gene Names: TXA2-R
• UniProt Entry Name: TA2R_HUMAN

NCBI Description

This gene encodes a member of the G protein-coupled receptor family. The protein interacts with thromboxane A2 to induce platelet aggregation and regulate hemostasis. A mutation in this gene results in a bleeding disorder. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

TXA2-R: Receptor for thromboxane A2 (TXA2), a potent stimulator of platelet aggregation. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. In the kidney, the binding of TXA2 to glomerular TP receptors causes intense vasoconstriction. Activates phospholipase C. Isoform 1 activates adenylyl cyclase. Isoform 2 inhibits adenylyl cyclase. Defects in TBXA2R are the cause of susceptibility to bleeding disorder platelet-type 13 (BDPLT13). BDPLT13 is a disorder characterized by reduced platelet aggregation and a tendency to mild mucocutaneous bleeding. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: integral to plasma membrane; acrosome; plasma membrane

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; thromboxane A2 receptor activity

Biological Process: second-messenger-mediated signaling; response to drug; platelet activation; response to lipopolysaccharide; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; elevation of cytosolic calcium ion concentration; positive regulation of vasoconstriction; positive regulation of smooth muscle contraction; inflammatory response; blood coagulation; positive regulation of blood pressure; response to nutrient; positive regulation of GTPase activity