Human proteasome (prosome, macropain) subunit, beta type, 2 ELISA Kit | PSMB2 elisa kit

Human proteasome (prosome, macropain) subunit, beta type, 2, PSMB2 ELISA Kit

Cat. Num. AAA914720

• Gene Names: PSMB2; HC7-I
• Reactivity: Human
• Synonyms: proteasome (prosome; macropain) subunit; beta type; 2; Human proteasome (prosome; PSMB2 ELISA Kit; 2 (PSMB2) ELISA kit; RP5-983H21.1; HC7-I; MGC104215; MGC126885; macropain subunit C7-I; multicatalytic endopeptidase complex subunit C7-1; proteasome beta 2 subunit; proteasome component C7-I; proteasome subunit; ; PSMB2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human PSMB2. No significant cross-reactivity or interference between human PSMB2 and analogues was observed.
• Sequence Length: 201

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 25 pg/ml -1600 pg/ml.
• Sensitivity: The minimum detectable dose of human PSMB2 is typically less than 6.2 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSMB2 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PSMB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PSMB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSMB2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSMB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4506195
• NCBI GeneID: 5690
• NCBI Accession #: NP_002785.1
• NCBI GenBank Nucleotide #: NM_002794.4
• UniProt Accession #: P49721; P31145; Q9BWZ9; D3DPS0
• Molecular Weight: 22,836 Da
• NCBI Official Full Name: proteasome subunit beta type-2 isoform 1
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, beta type, 2
• NCBI Official Symbol: PSMB2
• NCBI Official Synonym Symbols: HC7-I
• NCBI Protein Information: proteasome subunit beta type-2; macropain subunit C7-I; proteasome beta 2 subunit; proteasome component C7-I; proteasome subunit, beta type, 2; multicatalytic endopeptidase complex subunit C7-1; multicatalytic endopeptidase complex subunit C7-I
• UniProt Protein Name: Proteasome subunit beta type-2
• Protein Family: Proteasome
• UniProt Gene Name: PSMB2
• UniProt Entry Name: PSB2_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

PSMB2: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit has a trypsin-like activity. Belongs to the peptidase T1B family.

Protein type: Proteasome complex; Protease; Cell cycle regulation; EC 3.4.25.1

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; membrane; cytosol; nucleus

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; response to organic nitrogen; response to organic cyclic substance; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis