Thyroid Peroxidase (TPO) Recombinant Protein | TPO recombinant protein

Recombinant Thyroid Peroxidase (TPO)

Cat. Num. AAA2009932

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Thyroid Peroxidase (TPO); Recombinant Thyroid Peroxidase (TPO); TPO recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-RTGPL FACIIGKQMK ALRDGDRFWW ENTNVFTDAQ RQELEKHSLP RVICDNTGLT RVPVDAFRIG KFPQDFESCE DIPSMDLELW RETFPQDDKC VFPEEVDNGN FVHCEESGKL VLVYSCFHGY KLQGQEQVTC TQKGWDSEPP VCKDVNECAD LTHPPCHPSA QCKNTKGSFQ CVCTDPYVLG EDEKTCIDSG RL
• Sequence Length: 914

• Applicable Applications for TPO recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Arg636~Leu832 (Accession # P35419) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6678417
• NCBI GeneID: 22018
• NCBI Accession #: NP_033443.1
• NCBI GenBank Nucleotide #: NM_009417.2
• UniProt Accession #: P35419; Q8C8B1
• Molecular Weight: 23.9kDa
• NCBI Official Full Name: thyroid peroxidase
• NCBI Official Synonym Full Names: thyroid peroxidase
• NCBI Official Symbol: Tpo
• NCBI Protein Information: thyroid peroxidase
• UniProt Protein Name: Thyroid peroxidase
• Protein Family: Thyroid peroxidase
• UniProt Gene Name: Tpo
• UniProt Synonym Gene Names: TPO
• UniProt Entry Name: PERT_MOUSE

NCBI Description

This gene encodes a membrane-bound glycoprotein. The encoded enzyme plays a central role in thyroid gland function. The enzyme functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mice with homozygous missense mutations in this gene exhibit hypothyroid dwarfism and hearing impairment. [provided by RefSeq, Sep 2015]

Uniprot Description

TPO: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease. Defects in TPO are the cause of thyroid dyshormonogenesis 2A (TDH2A). A disorder due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. Belongs to the peroxidase family. XPO subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 1.11.1.8; Amino Acid Metabolism - tyrosine; Oxidoreductase; Mitochondrial

Cellular Component: extracellular space; cell surface; membrane; mitochondrion; plasma membrane; integral to membrane

Molecular Function: peroxidase activity; metal ion binding; heme binding; iodide peroxidase activity; calcium ion binding; oxidoreductase activity

Biological Process: embryonic hemopoiesis; hydrogen peroxide catabolic process; thyroid hormone generation; response to oxidative stress; hormone biosynthetic process