Thyroid Peroxidase (TPO) Recombinant Protein | TPO recombinant protein

Recombinant Thyroid Peroxidase (TPO)

Cat. Num. AAA2008999

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Thyroid Peroxidase (TPO); Recombinant Thyroid Peroxidase (TPO); TPO recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-QFST DALSADILAT IANLSGCLPF MLPPRCPDTC LANKYRPITG VCNNRDHPRW GASNTALARW LPPVYEDGFS QPRGWNPNFL YHGFPLPPVR EVTRHLIQVS NEAVTEDDQY SDFLPVWGQY IDHDIALTPQ STSTAAFWGG VDCQLTCENQ NPCFPIQLPS NSSRTT
• Sequence Length: 914

• Applicable Applications for TPO recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Gln107~Thr276 (Accession # P14650) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 129832
• NCBI GeneID: 54314
• UniProt Accession #: P14650
• Molecular Weight: 20.5kDa
• NCBI Official Full Name: Thyroid peroxidase
• NCBI Official Synonym Full Names: thyroid peroxidase
• NCBI Official Symbol: Tpo
• NCBI Protein Information: thyroid peroxidase
• UniProt Protein Name: Thyroid peroxidase
• Protein Family: Thyroid peroxidase
• UniProt Gene Name: Tpo
• UniProt Synonym Gene Names: TPO
• UniProt Entry Name: PERT_RAT

NCBI Description

membrane-bound glycoprotein; iodinates tyrosine residues in thyroglobulin to generate biologically active thyroid hormones T3 and T4 [RGD, Feb 2006]

Uniprot Description

Function: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.

Catalytic activity: 2 iodide + H2O2 + 2 H+ = 2 iodine + 2 H2O.[Thyroglobulin]-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3-iodo-L-tyrosine + 2 H2O.[Thyroglobulin]-3-iodo-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3,5-diiodo-L-tyrosine + 2 H2O.2 [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-L-thyroxine + [thyroglobulin]-aminoacrylate + 2 H2O.[Thyroglobulin]-3-iodo-L-tyrosine + [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-3,5,3'-triiodo-L-thyronine + [thyroglobulin]-aminoacrylate + 2 H2O.

Cofactor: Binds 1 calcium ion per heterodimer

By similarity.Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer

By similarity.

Pathway: Hormone biosynthesis; thyroid hormone biosynthesis.

Subunit structure: Interacts with DUOX1, DUOX2 and CYBA

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein

By similarity.

Post-translational modification: Heme is covalently bound through a H2O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface

By similarity.Cleaved in its N-terminal part

By similarity.

Sequence similarities: Belongs to the peroxidase family. XPO subfamily.Contains 1 EGF-like domain.Contains 1 Sushi (CCP/SCR) domain.