Growth Hormone Releasing Hormone (GHRH) Recombinant Protein | GHRH recombinant protein

Recombinant Growth Hormone Releasing Hormone (GHRH)

Cat. Num. AAA2012178

• Gene Names: Ghrh; Ghrf
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Growth Hormone Releasing Hormone (GHRH); Recombinant Growth Hormone Releasing Hormone (GHRH); GHRH recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-L PPSPPFRMQR HVDAIFTTNY RKLLSQLYAR KVIQDIMNKQ GERIQEQRAR LSRQEDSMWT EDKQMTLESI LQGFPRMKPS ADA
• Sequence Length: 103

• Applicable Applications for GHRH recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Leu20~Ala103 (Accession # P16043) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6753984
• NCBI GeneID: 14601
• NCBI Accession #: NP_034415.1
• NCBI GenBank Nucleotide #: NM_010285.2
• UniProt Accession #: P16043
• Molecular Weight: 11.5kDa
• NCBI Official Full Name: somatoliberin
• NCBI Official Synonym Full Names: growth hormone releasing hormone
• NCBI Official Symbol: Ghrh
• NCBI Official Synonym Symbols: Ghrf
• NCBI Protein Information: somatoliberin; GRF; growth hormone-releasing factor; growth hormone-releasing hormone
• UniProt Protein Name: Somatoliberin
• Protein Family: Somatoliberin
• UniProt Gene Name: Ghrh
• UniProt Synonym Gene Names: GRF; GHRH
• UniProt Entry Name: SLIB_MOUSE

Uniprot Description

GHRH: GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone. Belongs to the glucagon family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; neuron projection; cell; extracellular region; terminal button; perikaryon; nerve terminal; intracellular; cytosol

Molecular Function: protein binding; growth hormone-releasing hormone activity; hormone activity; growth hormone-releasing hormone receptor binding

Biological Process: response to food; positive regulation of transcription, DNA-dependent; positive regulation of hormone secretion; positive regulation of multicellular organism growth; positive regulation of cAMP metabolic process; G-protein signaling, adenylate cyclase activating pathway; positive regulation of growth hormone secretion; G-protein signaling, coupled to cAMP nucleotide second messenger; growth hormone secretion; elevation of cytosolic calcium ion concentration; cAMP-mediated signaling; circadian sleep/wake cycle, non-REM sleep; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; adenohypophysis development; positive regulation of circadian sleep/wake cycle, non-REM sleep