Typical Testing Data/Standard Curve (for reference only)
Mouse Interferon Alpha 4 ELISA Kit | IFNa4 elisa kit
Mouse IFNa4 (Interferon Alpha 4) ELISA Kit
Gene Names
IFNA4; INFA4; IFN-alpha4a
Reactivity
Mouse
Synonyms
Interferon Alpha 4; Mouse IFNa4 (Interferon Alpha 4) ELISA Kit; IFNa4 elisa kit
Reactivity
Mouse
Specificity
This kit recognizes Mouse IFNalpha4 in samples. No significant cross-reactivity or interference between Mouse IFNalpha4 and analogues was observed.
Sequence Length
189
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
31.25-2000pg/mL
Sensitivity
18.75pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IFNalpha4 were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IFNalpha4 were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IFNa4 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse IFNalpha4 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFNalpha4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFNalpha4 and Avidin- Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFNalpha4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse IFNalpha4. You can calculate the concentration of Mouse IFNalpha4 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFNalpha4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFNalpha4 and Avidin- Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFNalpha4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse IFNalpha4. You can calculate the concentration of Mouse IFNalpha4 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,808 Da
NCBI Official Full Name
interferon alpha-4
NCBI Official Synonym Full Names
interferon, alpha 4
NCBI Official Symbol
IFNA4
NCBI Official Synonym Symbols
INFA4; IFN-alpha4a
NCBI Protein Information
interferon alpha-4
UniProt Protein Name
Interferon alpha-4
UniProt Gene Name
IFNA4
UniProt Synonym Gene Names
IFN-alpha-4
UniProt Entry Name
IFNA4_HUMAN
Uniprot Description
IFNA4: Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. Belongs to the alpha/beta interferon family.
Protein type: Cytokine; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 9p22
Cellular Component: extracellular region; extracellular space
Molecular Function: cytokine activity; interferon-alpha/beta receptor binding; protein binding
Biological Process: adaptive immune response; B cell differentiation; B cell proliferation; blood coagulation; cytokine and chemokine mediated signaling pathway; defense response to virus; humoral immune response; innate immune response; natural killer cell activation during immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to exogenous dsRNA; response to virus; T cell activation during immune response
Research Articles on IFNa4
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Product Notes
The Mouse IFNa4 ifna4 (Catalog #AAA2533516) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533516 ELISA Kit recognizes Mouse IFNa4. It is sometimes possible for the material contained within the vial of "Interferon Alpha 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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