ELISA (EIA)
(Sandwich ELISA analysis of human IFN gamma using Mouse anti Human IFN gamma as a capture reagent and biotinylated Mouse anti Human IFN gamma (MBS226157) as a detection reagent with purified recombinant IFN gamma as the antigen. Detection was pe)
Mouse anti-Human IFN Gamma Monoclonal Antibody | anti-IFNGAMMA antibody
Mouse anti Human IFN Gamma:Biotin
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG conjugated to Biotin - liquid
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
ELISA (EIA)
(Sandwich ELISA analysis of human IFN gamma using Mouse anti Human IFN gamma as a capture reagent and biotinylated Mouse anti Human IFN gamma (MBS226157) as a detection reagent with purified recombinant IFN gamma as the antigen. Detection was pe)
ELISA (EIA)
(Sandwich ELISA analysis of human IFN gamma using Mouse anti Human IFN gamma as a capture reagent and biotinylated Mouse anti Human IFN gamma (MBS226157) as a detection reagent with purified recombinant IFN gamma as the antigen. Detection was pe)
After infection antigen presenting cells secrete IL-12, IL-18 and IL-1 beta which can promote IFN gamma synthesis (Schoenborn & Wilson 2007). IFN gamma can then elicit responses through interferon gamma receptor 1 and interferon gamma receptor 2. Activation of these receptors can then trigger immune responses through the JAK/STAT signaling pathway (Pereiro et al. 2019).
Under physiological conditions, IFN gamma is constitutively expressed through a tightly controlled mechanism. However, it has been shown to have some protumorigenic roles, by upregulating checkpoint inhibitor programmed cell-death ligand 1. Conversely though, there have been some positive associations between IFN gamma and patient survival in several cancer types. As such, the exact roles that IFN gamma plays in cancer may largely be dependent on tumor specific context and microenvironmental cues (Castro et al. 2018).
The biotinylated Mouse anti Human IFN gamma antibody, clone 18F11G6 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human IFN gamma antibody, clone 8C4H9 as the capture antibody.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]
Uniprot Description
IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.
Protein type: Cytokine; Secreted; Membrane protein, integral; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 12q14
Cellular Component: extracellular space; extracellular region; external side of plasma membrane
Molecular Function: interferon-gamma receptor binding; cytokine activity
Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; positive regulation of osteoclast differentiation; negative regulation of smooth muscle cell proliferation; apoptosis; positive regulation of interleukin-23 production; positive regulation of interleukin-12 production; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of killing of cells of another organism; sensory perception of mechanical stimulus; positive regulation of interleukin-1 beta secretion; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; neutrophil chemotaxis; positive regulation of synaptic transmission, cholinergic; adaptive immune response; negative regulation of myelination; CD8-positive, alpha-beta T cell differentiation during immune response; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; positive regulation of tumor necrosis factor production; defense response to protozoan; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of chemokine biosynthetic process; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; cell motility; regulation of insulin secretion
Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Mycobacterium Tuberculosis, Susceptibility To; Tuberous Sclerosis 2; Human Immunodeficiency Virus Type 1, Susceptibility To
Research Articles on IFNGAMMA
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Product Notes
The IFNGAMMA ifng (Catalog #AAA226157) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human IFN Gamma:Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IFN Gamma can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 1.0ug/ml. Researchers should empirically determine the suitability of the IFNGAMMA ifng for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFN Gamma, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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