Toll Interacting Protein (TOLLIP) Recombinant Protein | TOLLIP recombinant protein

Recombinant Toll Interacting Protein (TOLLIP)

Cat. Num. AAA2009550

• Gene Names: Tollip; 4930403G24Rik; 4931428G15Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Toll Interacting Protein (TOLLIP); Recombinant Toll Interacting Protein (TOLLIP); TOLLIP recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-QD FLRITPTQQQ QQIQLDAQAA QQLQYGGTVG TVGRLSITVV QAKLAKNYGM TRMDPYCRLR LGYAVYETPT AHNGAKNPRW NKVIQCTVPP GVDSFYLEIF DERAFSMDDR IAWTHITIPE SLKQGQVEDE WYSLSGRQGD DKEGMINLVM SYTSLPAAMM MPPQPVVLMP TVYQQGVGYV PIAGMPAVCS PGMVPMAMPP PAVAPQPRCN EEDLKAIQDM FPNMDREVIR SVLE
• Sequence Length: 274

• Applicable Applications for TOLLIP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Gln19~Glu254 (Accession # Q9QZ06) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cytoplasm
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.1
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 13591860
• NCBI GeneID: 54473
• NCBI Accession #: NP_076253.1
• NCBI GenBank Nucleotide #: NM_023764.3
• UniProt Accession #: Q9QZ06; Q543N1; Q9D5P5
• Molecular Weight: 30.1kDa
• NCBI Official Full Name: toll-interacting protein
• NCBI Official Synonym Full Names: toll interacting protein
• NCBI Official Symbol: Tollip
• NCBI Official Synonym Symbols: 4930403G24Rik; 4931428G15Rik
• NCBI Protein Information: toll-interacting protein
• UniProt Protein Name: Toll-interacting protein
• Protein Family: Toll-interacting protein
• UniProt Gene Name: Tollip
• UniProt Entry Name: TOLIP_MOUSE

Uniprot Description

TOLLIP: Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Oligomerizes. Binds to TLR2 and the TLR4-MD2 complex via its C-terminus. Exists as complex with IRAK1 in unstimulated cells. Upon IL-1 signaling, Tollip binds to the activated IL-1 receptor complex containing IL-1RI, IL-1RacP and the adapter protein MyD88, where it interacts with the TIR domain of IL-1RacP. MyD88 then triggers IRAK1 autophosphorylation, which in turn leads to the dissociation of IRAK1 from Tollip and IL-1RAcP. Interacts with TOM1L2. Belongs to the tollip family.

Protein type: Inhibitor; Vesicle

Cellular Component: nuclear body; perinuclear region of cytoplasm; cytoplasm

Molecular Function: protein binding; SUMO binding; ubiquitin conjugating enzyme binding; ubiquitin protein ligase binding; interleukin-1, Type I receptor binding; kinase binding

Biological Process: positive regulation of protein sumoylation; immune system process; autophagy; innate immune response; signal transduction; inflammatory response; phosphorylation