Ephrin Type A Receptor 1 (EPHA1) Recombinant Protein | EPHA1 recombinant protein

Recombinant Ephrin Type A Receptor 1 (EPHA1)

Cat. Num. AAA2018240

• Gene Names: Epha1; Eph; Esk; AL033318; 5730453L17Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Ephrin Type A Receptor 1 (EPHA1); Recombinant Ephrin Type A Receptor 1 (EPHA1); EPHA1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KERED QLAPGQLVAM LLGIASGMNC LSGHNYVHRD LAARNILVNQ NLCCKVSDFG LTRLLDDFDG TYETQGGKIP IRWTAPEAIA HRIFTTASDV WSFGIVMWEV LSFGDKPYGE MSNQEVMKSI EDGYRLPPPV DCPAPLYELM KNCWAYDRAR RPHFLQLQAH LEQLLTDPHS LRTIANFDPR VTLRLPSLSGSDGIPYRSVS EWLESIRMKR YILHFRSAGL DTMECVLELT AEDLTQMGIT
• Sequence Length: 977

• Applicable Applications for EPHA1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Lys716~Thr960 (Accession # Q60750) with two N-terminal Tags, His-tag and T7-tag
• Subcellular Location: Cell membrane; Single-pass type I membrane protein
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.1
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 31980651
• NCBI GeneID: 13835
• NCBI Accession #: NP_076069.2
• NCBI GenBank Nucleotide #: NM_023580.4
• UniProt Accession #: Q60750; Q8CED9; Q9ESJ2
• Molecular Weight: 31.6kDa
• NCBI Official Full Name: ephrin type-A receptor 1
• NCBI Official Synonym Full Names: Eph receptor A1
• NCBI Official Symbol: Epha1
• NCBI Official Synonym Symbols: Eph; Esk; AL033318; 5730453L17Rik
• NCBI Protein Information: ephrin type-A receptor 1; mEpha1; embryonic stem cell kinase; tyrosine-protein kinase receptor ESK
• UniProt Protein Name: Ephrin type-A receptor 1
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: Epha1
• UniProt Synonym Gene Names: Esk; mEpha1
• UniProt Entry Name: EPHA1_MOUSE

Uniprot Description

EphA1: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds with a low affinity to ephrin-A1. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Misexpressed in several cancers, including upregulation in head and neck cancer, and downregulation in invasive breast cancer cell lines and glioblastoma

Protein type: Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; Eph family

Cellular Component: membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: transferase activity; transmembrane-ephrin receptor activity; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; ephrin receptor activity; nucleotide binding; transmembrane receptor protein tyrosine kinase activity; kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; positive regulation of angiogenesis; cell surface receptor linked signal transduction; positive regulation of stress fiber formation; positive regulation of cell proliferation; ephrin receptor signaling pathway; negative regulation of protein kinase activity; angiogenesis; cell adhesion; positive regulation of cell-matrix adhesion; phosphorylation; negative regulation of cell migration; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration