Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) Recombinant Protein | CTLA4 recombinant protein

Recombinant Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4)

Cat. Num. AAA2018426

• Gene Names: Ctla4; Cd152; CTLA-4; sCTLA4
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4); Recombinant Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4); CTLA4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TQPSVVLASS HGVASFPCEY ASSHNTDEVR VTVLRQTNDQ VTEVCATTFT VKNTLGFLDD PFCSGTFNES RVNLTIQGLR AADTGLYFCK VELMYPPPYF VGMGNGTQIY VIDPEPCPDS DFLLWILAAV SSGLFFYSFLVTAVSLNRTL KK
• Sequence Length: 223

• Applicable Applications for CTLA4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Thr41~Lys192 (Accession # Q62859) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.1
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 13928934
• NCBI GeneID: 63835
• NCBI Accession #: NP_113862.1
• NCBI GenBank Nucleotide #: NM_031674.1
• UniProt Accession #: Q62859
• Molecular Weight: 20.5kDa
• NCBI Official Full Name: cytotoxic T-lymphocyte protein 4
• NCBI Official Synonym Full Names: cytotoxic T-lymphocyte-associated protein 4
• NCBI Official Symbol: Ctla4
• NCBI Official Synonym Symbols: Cd152; CTLA-4; sCTLA4
• NCBI Protein Information: cytotoxic T-lymphocyte protein 4; soluble form; CD152 antigen; transmembrane form
• UniProt Protein Name: Cytotoxic T-lymphocyte associated protein-4
• Protein Family: Cytotoxic T-lymphocyte protein
• UniProt Gene Name: Ctla4
• UniProt Synonym Gene Names: CTLA-4
• UniProt Entry Name: Q62859_RAT

NCBI Description

immunoregulatory receptor expressed on the surface of T and B lymphocytes [RGD, Feb 2006]

Uniprot Description

CTLA-4: Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. Genetic variation in CTLA4 influences susceptibility to systemic lupus erythematosus (SLE). SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Genetic variations in CTLA4 may influence susceptibility to Graves disease, an autoimmune disorder associated with overactivity of the thyroid gland and hyperthyroidism. Genetic variation in CTLA4 is the cause of susceptibility to diabetes mellitus insulin-dependent type 12 (IDDM12). A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. Genetic variation in CTLA4 is the cause of susceptibility to celiac disease type 3 (CELIAC3). It is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Immunoglobulin superfamily

Cellular Component: Golgi apparatus; membrane; perinuclear region of cytoplasm; clathrin-coated endocytic vesicle; external side of plasma membrane

Molecular Function: protein binding; receptor activity

Biological Process: B cell receptor signaling pathway; negative regulation of T cell proliferation; negative regulation of regulatory T cell differentiation; positive regulation of apoptosis; negative regulation of immune response; immune response; negative regulation of B cell proliferation; response to DNA damage stimulus