LIGHT Recombinant Protein | TNFSF14 recombinant protein

LIGHT Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123073

• Gene Names: Tnfsf14; LTg; HVEML; LIGHT; Ly113; HVEM-L
• Purity: >95% as determined by SDS-PAGE
• Synonyms: LIGHT; LIGHT Protein; Mouse; Recombinant (His Tag); Mouse TNFSF14/LIGHT/CD258 Protein (His Tag); HVEM-L Protein; HVEML Protein; LTg Protein; Ly113 Protein; tumor necrosis factor (ligand) superfamily; member 14; TNFSF14 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, pH8.0, 500mM NaCl, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Asp72-Val239

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mouse TNFSF14 (NP_062291.1)(Asp72-Val239) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TNFSF14 recombinant protein

Background: LIGHT, also known as TNFSF14 or CD258, is a newly identified member of the TNF superfamily (TNFSF14) that is expressed by activated T lymphocytes, monocytes, granulocytes, spleen cells, and immature dendritic cells. TNFSF14/LIGHT/CD258 is a type II transmembrane protein that is known to bind 2 membrane-bound TNFSF signaling receptors: HVEM, which is predominantly expressed by T cells, and lymphotoxin beta receptor (LTbetaR), which is expressed by stromal cells and nonlymphoid hematopoietic cells. TNFSF14/LIGHT/CD258 also binds to a soluble non-signaling receptor, decoy receptor 3 (DcR3), which can modulate the function of LIGHT in vivo. TNFSF14/LIGHT/CD258 can also costimulate T cell responses via HVEM, which is constitutively expressed in most lymphocyte subpopulations, including CD4⁺ and CD⁸⁺ T cells. In addition, TNFSF14/LIGHT/CD258 has been shown to suppress tumor formation in vivo and to induce tumor cell apoptosis via the up-regulation of intercellular adhesion molecule 1 and an increased lymphocyte adhesion to cancer cells. Thus, TNFSF14/LIGHT/CD258 is being actively investigated as a possible basis for cancer treatment.

Product Categories/Family for TNFSF14 recombinant protein

Cancer Drug Targets

References

Ogawa T, et al. (2010) CXCR3 binding chemokine and TNFSF14 over expression in bladder urothelium of patients with ulcerative interstitial cystitis. J Urol. 183(3): 1206-12.Kanodia S, et al. (2010) Expression of LIGHT/TNFSF14 combined with vaccination against human papillomavirus Type 16 E7 induces significant tumor regression. Cancer Res. 70(10): 3955-64.Hosokawa Y, et al. (2010) TNFSF14 coordinately enhances CXCL10 and CXCL11 productions from IFN-gamma-stimulated human gingival fibroblasts. Mol Immunol. 47(4): 666-70.

NCBI and Uniprot Product Information

• NCBI GI #: 9507195
• NCBI GeneID: 50930
• NCBI Accession #: NP_062291.1
• NCBI GenBank Nucleotide #: NM_019418.3
• UniProt Accession #: Q9QYH9; Q059Y9
• Molecular Weight: 26,338 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 14
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 14
• NCBI Official Symbol: Tnfsf14
• NCBI Official Synonym Symbols: LTg; HVEML; LIGHT; Ly113; HVEM-L
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 14
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 14
• Protein Family: Light-induced protein
• UniProt Gene Name: Tnfsf14
• UniProt Synonym Gene Names: Light
• UniProt Entry Name: TNF14_MOUSE

Uniprot Description

TNFSF14: Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Inhibitor; Membrane protein, integral

Cellular Component: extracellular space; membrane; integral to membrane; extracellular region; plasma membrane

Molecular Function: caspase inhibitor activity; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: release of cytoplasmic sequestered NF-kappaB; T cell costimulation; immune response; negative regulation of caspase activity; positive regulation of myoblast differentiation