TNFSF14 / LIGHT / CD258 recombinant protein

Recombinant Human TNFSF14 / LIGHT / CD258 Protein (His tag)

Cat. Num. AAA2546291

• Gene Names: TNFSF14; LTg; CD258; HVEML; LIGHT
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: TNFSF14 / LIGHT / CD258; Recombinant Human TNFSF14 / LIGHT / CD258 Protein (His tag); CD258; HVEML; LIGHT; LTg; TR2; TNFSF14 / LIGHT / CD258 recombinant protein

• Host: Human Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 240

• Application Notes: The recombinant human TNFSF14 consists of 183 amino acids and and has a predicted molecular mass of 20.4 kDa.
• Predicted N Terminal: His
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TNFSF14 / LIGHT / CD258 recombinant protein

Background: LIGHT, also known as TNFSF14 or CD258, is a newly identified member of the TNF superfamily (TNFSF14) that is expressed by activated T lymphocytes, monocytes, granulocytes, spleen cells, and immature dendritic cells. TNFSF14 / LIGHT / CD258 is a type II transmembrane protein that is known to bind 2 membrane-bound TNFSF signaling receptors: HVEM, which is predominantly expressed by T cells, and lymphotoxin beta receptor (LTbetaR), which is expressed by stromal cells and nonlymphoid hematopoietic cells. TNFSF14 / LIGHT / CD258 also binds to a soluble nonsignaling receptor, decoy receptor 3 (DcR3), which can modulate the function of LIGHT in vivo. TNFSF14 / LIGHT / CD258 can also costimulate T cell responses via HVEM, which is constitutively expressed in most lymphocyte subpopulations, including CD4+ and CD8+ T cells. In addition, TNFSF14 / LIGHT / CD258 has been shown to suppress tumor formation in vivo and to induce tumor cell apoptosis via the up-regulation of intercellular adhesion molecule 1 and an increased lymphocyte adhesion to cancer cells. Thus, TNFSF14 / LIGHT / CD258 is being actively investigated as a possible basis for cancer treatment.

Description: A DNA sequence encoding the human TNFSF14 (NP_003798.2) extracellular domain (Asp 74-Val 240) was fused with a polyhistidine-tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 25952144
• NCBI GeneID: 8740
• NCBI Accession #: NP_003798.2
• NCBI GenBank Nucleotide #: NM_003807.4
• UniProt Accession #: O43557; O75476; Q6FHA1; Q8WVF8; Q96LD2; A8K7M2; C9J5H4
• Molecular Weight: 22,395 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 14 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor superfamily member 14
• NCBI Official Symbol: TNFSF14
• NCBI Official Synonym Symbols: LTg; CD258; HVEML; LIGHT
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 14
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 14
• UniProt Gene Name: TNFSF14
• UniProt Synonym Gene Names: HVEML; LIGHT; HVEM-L; Herpesvirus entry mediator ligand

NCBI Description

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFSF14: Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Activates NFKB, stimulates the proliferation of T-cells, and inhibits growth of the adenocarcinoma HT-29. Acts as a receptor for Herpes simplex virus. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Inhibitor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: plasma membrane

Molecular Function: caspase inhibitor activity; protein binding; receptor binding

Biological Process: apoptosis; release of cytoplasmic sequestered NF-kappaB; tumor necrosis factor-mediated signaling pathway