LIGHT Active Protein | TNFSF14 active protein

LIGHT Protein, Human, Recombinant (rFc Tag)

Cat. Num. AAA8120242

• Purity: >90% as determined by SDS-PAGE
• Synonyms: LIGHT; LIGHT Protein; Human; Recombinant (rFc Tag); Human TNFSF14/LIGHT/CD258 Protein (Fc Tag); CD258 Protein; HVEML Protein; LTg Protein; TR2 Protein; tumor necrosis factor (ligand) superfamily; member 14; TNFSF14 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH8.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Asp74-Val240

• Species: Human
• Activity: Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 5-25ng/mL.
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Ser
• Tag: N-rabbit IgG-Fc
• Protein Construction: A DNA sequence encoding the human TNFSF14 (AAK26160.1)(Asp74-Val240) was expressed with the Fc region of rabbit IgG at the N-terminus..
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 5-25 ng/mL.)

Related Product Information for TNFSF14 active protein

Background: LIGHT, also known as TNFSF14 or CD258, is a newly identified member of the TNF superfamily (TNFSF14) that is expressed by activated T lymphocytes, monocytes, granulocytes, spleen cells, and immature dendritic cells. TNFSF14/LIGHT/CD258 is a type II transmembrane protein that is known to bind 2 membrane-bound TNFSF signaling receptors: HVEM, which is predominantly expressed by T cells, and lymphotoxin beta receptor (LTbetaR), which is expressed by stromal cells and nonlymphoid hematopoietic cells. TNFSF14/LIGHT/CD258 also binds to a soluble non-signaling receptor, decoy receptor 3 (DcR3), which can modulate the function of LIGHT in vivo. TNFSF14/LIGHT/CD258 can also costimulate T cell responses via HVEM, which is constitutively expressed in most lymphocyte subpopulations, including CD4⁺ and CD⁸⁺ T cells. In addition, TNFSF14/LIGHT/CD258 has been shown to suppress tumor formation in vivo and to induce tumor cell apoptosis via the up-regulation of intercellular adhesion molecule 1 and an increased lymphocyte adhesion to cancer cells. Thus, TNFSF14/LIGHT/CD258 is being actively investigated as a possible basis for cancer treatment.

Product Categories/Family for TNFSF14 active protein

Cancer Drug Targets

References

Ogawa T, et al. (2010) CXCR3 binding chemokine and TNFSF14 over expression in bladder urothelium of patients with ulcerative interstitial cystitis. J Urol. 183(3): 1206-12.Kanodia S, et al. (2010) Expression of LIGHT/TNFSF14 combined with vaccination against human papillomavirus Type 16 E7 induces significant tumor regression. Cancer Res. 70(10): 3955-64.Hosokawa Y, et al. (2010) TNFSF14 coordinately enhances CXCL10 and CXCL11 productions from IFN-gamma-stimulated human gingival fibroblasts. Mol Immunol. 47(4): 666-70.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant human TNFSF14 consists of 418 amino acids and predicts a molecular mass of 45.7kDa.
• Protein Family: Light-induced protein