Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) Recombinant Protein | TRAIL recombinant protein

Recombinant Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)

Cat. Num. AAA2009976

• Gene Names: Tnfsf10; TL2; Ly81; Trail; APO-2L; AI448571; A330042I21Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL); Recombinant Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL); TRAIL recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MHHHHHHSSGLVPRGSGMKETAAAKF ERQHMDSPDLGTDDDDKAMADIGSEF-M YFTNEMKQLQ DNYSKIGLAC FSKTDEDFWD STDGEILNRP CLQVKRQLYQ LIEEVTLRTF QDTISTVPEK QLSTPPLPRG GRPQKVAAHI TGITRRSNSA LIPISKDGKT LGQKIESWES SRKGHSFLNH VLFRNGELVI EQEGLYYIYS QTYFRFQEAE DASKMVSKDK VRTKQLVQYI YKYTSYPDPI VLMKSARNSC WSRDAEYGLY SIYQGGLFEL KKNDRIFVSV TNEHLMDLDQ EASFFGAFLI N
• Sequence Length: 291

• Applicable Applications for TRAIL recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met40~Asn291 (Accession # P50592) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 6678431
• NCBI GeneID: 22035
• NCBI Accession #: NP_033451.1
• NCBI GenBank Nucleotide #: NM_009425.2
• UniProt Accession #: P50592
• Molecular Weight: 34.9kDa
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 10
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 10
• NCBI Official Symbol: Tnfsf10
• NCBI Official Synonym Symbols: TL2; Ly81; Trail; APO-2L; AI448571; A330042I21Rik
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 10; TRAIL/APO2L; TNF-related apoptosis inducing ligand; TNF-related apoptosis-inducing ligand
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 10
• UniProt Gene Name: Tnfsf10
• UniProt Synonym Gene Names: Trail; Protein TRAIL
• UniProt Entry Name: TNF10_MOUSE

Uniprot Description

TRAIL: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. Homotrimer. Widespread; most predominant in spleen, lung and prostate. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cytokine

Cellular Component: extracellular space; membrane; integral to membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding

Biological Process: positive regulation of apoptosis; apoptosis; positive regulation of caspase activity; immune response