Mouse tumor necrosis factor (ligand) superfamily, member 10 ELISA Kit | TNFSF10 elisa kit

Mouse soluble tumor necrosis factor-related apoptosis inducing ligand, sTRAIL ELISA Kit

Cat. Num. AAA701538

• Gene Names: Tnfsf10; TL2; Ly81; Trail; APO-2L; AI448571; A330042I21Rik
• Reactivity: Mouse
• Synonyms: tumor necrosis factor (ligand) superfamily; member 10; Mouse soluble tumor necrosis factor-related apoptosis inducing ligand; sTRAIL ELISA Kit; sTRAIL ELISA KIT; APO2L; Apo-2L; CD253; TL2; TRAIL; Apo-2 ligand; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family; tumor necrosis factor apoptosis-inducing ligand splice var; TNFSF10 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse TNFSF10. No significant cross-reactivity or interference between Mouse TNFSF10 and analogues was observed.
• Sequence Length: 291

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 3.12 pg/ml-200 pg/ml
• Sensitivity: 0.78 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Apoptosis/Autophagy
• Protein Biological Process 3: Apoptosis
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNFSF10 elisa kit

Introduction: In the field of cell biology, TNF-related apoptosis-inducing ligand (TRAIL), is a protein which functions as a ligand which induces the process of cell death called apoptosis. TRAIL is a type II membrane protein of the TNF superfamily. Unlike other members of the TNF superfamily that interact with one or two specific receptors, TRAIL can potentially interact with five different receptors. TRAIL can induce apoptosis by interaction with two receptors, referred to as TRAIL-R1 (DR4) and TRAIL-R2 (DR5). These receptors have an intracellular death domain that mediates cellular apoptosis. Two other receptors, known as TRAIL-R3 and TRAIL-R4, inhibit apoptosis by acting as decoy receptors because they do not contain the cytoplasmic death domain. These decoy receptors are highly expressed in normal tissues but have substantially lower expression in malignant cells. Soluble TRAIL is unique in that it frequently induces apoptosis of transformed cells in vitro and in xenograft murine models without causing apoptosis of normal cells. TRAIL induces apoptosis in various human cancer cells, a promising observation because it raises the possibility of a death ligand with selectivity for tumor cells.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to sTRAIL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for sTRAIL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain sTRAIL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of sTRAIL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6678431
• NCBI GeneID: 22035
• NCBI Accession #: NP_033451.1
• NCBI GenBank Nucleotide #: NM_009425.2
• UniProt Accession #: P50592
• Molecular Weight: 33,477 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 10
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 10
• NCBI Official Symbol: Tnfsf10
• NCBI Official Synonym Symbols: TL2; Ly81; Trail; APO-2L; AI448571; A330042I21Rik
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 10; TRAIL/APO2L; TNF-related apoptosis inducing ligand; TNF-related apoptosis-inducing ligand
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 10
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: Tnfsf10
• UniProt Synonym Gene Names: Trail; Protein TRAIL
• UniProt Entry Name: TNF10_MOUSE

Uniprot Description

Function: Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.