TMP21 Recombinant Protein | TMED10 recombinant protein

TMP21 Protein, Human, Recombinant (mFc Tag)

Cat. Num. AAA8121418

• Gene Names: TMED10; p23; TMP21; S31I125; Tmp-21-I; S31III125; P24(DELTA)
• Purity: >85% as determined by SDS-PAGE
• Synonyms: TMP21; TMP21 Protein; Human; Recombinant (mFc Tag); Human TMP21/TMED10 Protein (Fc Tag); p23 Protein; P24(DELTA) Protein; S31I125 Protein; S31III125 Protein; Tmp-21-I Protein; transmembrane emp24-like trafficking protein 10 (yeast); TMED10 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg185

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Ile 32
• Tag: C-mouse IgG1-Fc
• Protein Construction: A DNA sequence encoding the human TMED10 (NP_006818.3)(Met1-Arg185) was expressed with the Fc region of mouse IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TMED10 recombinant protein

Background: TMED10 disrupts the complex formation between TGF-beta type I (also termed ALK5) and type II receptors (TbetaRII). Misexpression studies revealed that TMED10 attenuated TGF-beta-mediated signaling. A 20-amino acid-long region from Thr(91) to Glu(110) within the extracellular region of TMED10 was found to be crucial for TMED10 interaction with both ALK5 and TbetaRII. Synthetic peptides corresponding to this region inhibit both TGF-beta-induced Smad2 phosphorylation and Smad-dependent transcriptional reporter activity. In a xenograft cancer model, where previously TGF-beta was shown to elicit tumor-promoting effects, gain-of-function and loss-of-function studies for TMED10 revealed a decrease and increase in the tumor size, respectively. That TMED10 expression levels are the key determinant for efficiency of TGF-beta receptor complex formation and signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 98986464
• NCBI GeneID: 10972
• NCBI Accession #: NP_006818.3
• NCBI GenBank Nucleotide #: NM_006827.5
• UniProt Accession #: P49755; Q15602; Q16536; Q86TC2; Q86TS5; B2R605
• Molecular Weight: 24,976 Da
• NCBI Official Full Name: transmembrane emp24 domain-containing protein 10
• NCBI Official Synonym Full Names: transmembrane emp24-like trafficking protein 10 (yeast)
• NCBI Official Symbol: TMED10
• NCBI Official Synonym Symbols: p23; TMP21; S31I125; Tmp-21-I; S31III125; P24(DELTA)
• NCBI Protein Information: transmembrane emp24 domain-containing protein 10; 21 kDa transmembrane trafficking protein; 21 kDa transmembrane-trafficking protein; p24 family protein delta-1; p24delta; p24delta1; transmembrane protein Tmp21
• UniProt Protein Name: Transmembrane emp24 domain-containing protein 10
• Protein Family: Transmembrane protein
• UniProt Gene Name: TMED10
• UniProt Synonym Gene Names: TMP21; S31I125; p24delta1
• UniProt Entry Name: TMEDA_HUMAN

NCBI Description

This gene is a member of the EMP24/GP25L/p24 family and encodes a protein with a GOLD domain. This type I membrane protein is localized to the plasma membrane and golgi cisternae and is involved in vesicular protein trafficking. The protein is also a member of a heteromeric secretase complex and regulates the complex's gamma-secretase activity without affecting its epsilon-secretase activity. Mutations in this gene have been associated with early-onset familial Alzheimer's disease. This gene has a pseudogene on chromosome 8. [provided by RefSeq, Jul 2008]

Uniprot Description

TMED10: Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act togther with TMED2 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1. In COPI vesicle-mediated retrograde transport involved in the biogenesis of COPI vesicles and vesicle coat recruitment. On Golgi membranes, acts as primary receptor for ARF1-GDP which is involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2LR1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent of modulation of gamma-secretase activity). As part of the presenilin-dependent gamma-secretase complex regulates gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40 (Abeta40). Involved in organization of the Golgi apparatus. Predominantly homodimeric and to lesser extent monomeric in endoplasmic reticulum. Homodimer and monomer in endoplasmic reticulum-Golgi intermediate compartment and cis-Golgi network. Probably oligomerizes with other members of the EMP24/GP25L family such as TMED2, TMED7 and TMED9. Interacts with TMED2. Associates with the COPI vesicle coat (coatomer); TMED10:TMED2 heterotetramers are proposed to be involved in coatomer association. Interacts (via C-terminus) with COPG1; the interaction involves dimeric TMED10. Interacts with ARF1 (GDP- bound); the interaction probably involves a TMED10 oligomer. Interacts with SEC23A; indicative for an association of TMED10 with the COPII vesicle coat. Interacts with CD59, SEC24B, SEC24C and SEC24D. Interacts with MPPE1/PGAP5. Interacts with F2LR1. Interacts with KDELR2; the interaction is disrupted by KDELR2 ligand. Found in a complex composed at least of SURF4, TMED2 and TMED10. Associates with the presenilin- dependent gamma-secretase complex. Ubiquitous. Belongs to the EMP24/GP25L family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; zymogen granule membrane; cis-Golgi network; secretory granule membrane; endoplasmic reticulum; ER-Golgi intermediate compartment; integral to membrane; Golgi membrane; COPI-coated vesicle; trans-Golgi network transport vesicle; melanosome; plasma membrane

Molecular Function: protein binding; syntaxin binding; protein complex binding

Biological Process: response to alkaloid; COPII coating of Golgi vesicle; intracellular protein transport; regulated secretory pathway; vesicle targeting, to, from or within Golgi; ER to Golgi vesicle-mediated transport; COPI coating of Golgi vesicle; retrograde vesicle-mediated transport, Golgi to ER; kidney development; Golgi organization and biogenesis; protein oligomerization