Human Transketolase ELISA Kit | TKT elisa kit

Human Transketolase ELISA Kit

Cat. Num. AAA9425166

• Gene Names: TKT; TK; TKT1; SDDHD; HEL107; HEL-S-48
• Reactivity: Human
• Synonyms: Transketolase; Human Transketolase ELISA Kit; TK; TKT; 2.2.1.1; TKT elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human TK. No significant cross-reactivity or interference was observed.

• Samples: Serum, plasma, tissue homogenates and cell culture supernates and other biological fluids
• Detection Range: 78.1 pg/mL -5000 pg/mL
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TKT elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human transketolase,TK concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TK, biotinconjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507521
• NCBI GeneID: 7086
• NCBI Accession #: NP_001055.1
• NCBI GenBank Nucleotide #: NM_001064.3
• UniProt Accession #: P29401; Q8TBA3; Q96HH3; A8K089; B4DE31; E7EPA7
• Molecular Weight: 68,814 Da
• NCBI Official Full Name: transketolase isoform 1
• NCBI Official Synonym Full Names: transketolase
• NCBI Official Symbol: TKT
• NCBI Official Synonym Symbols: TK; TKT1; SDDHD; HEL107; HEL-S-48
• NCBI Protein Information: transketolase
• UniProt Protein Name: Transketolase
• Protein Family: Transketolase
• UniProt Gene Name: TKT
• UniProt Synonym Gene Names: TK

NCBI Description

This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

TKT: Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate. Homodimer. Belongs to the transketolase family.

Protein type: Carbohydrate Metabolism - pentose phosphate pathway; EC 2.2.1.1; Transferase

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: cytosol; nuclear body; nuclear speck; nucleoplasm; peroxisome; vesicle

Molecular Function: cofactor binding; protein binding; protein homodimerization activity; transketolase activity

Biological Process: glyceraldehyde-3-phosphate biosynthetic process; pentose-phosphate shunt; xylulose biosynthetic process

Disease: Short Stature, Developmental Delay, And Congenital Heart Defects