Mouse guanylate cyclase activator 2B (uroguanylin) ELISA Kit | GUCA2B elisa kit

Mouse guanylate cyclase activator 2B (uroguanylin) , GUCA2B ELISA Kit

Cat. Num. AAA912299

• Gene Names: Guca2b; Ugn; Gcap2; AV066530
• Reactivity: Mouse
• Synonyms: guanylate cyclase activator 2B (uroguanylin); Mouse guanylate cyclase activator 2B (uroguanylin); GUCA2B ELISA Kit; Mouse guanylate cyclase activator 2B (uroguanylin) (GUCA2B) ELISA kit; GCAP-II; UGN; guanylate cyclase activator 2B; uroguanylin; GUCA2B elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse GUCA2B. No significant cross-reactivity or interference between mouse GUCA2B and analogues was observed.
• Sequence Length: 106

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 7.8 pg/ml-500 pg/ml
• Sensitivity: < 1.95 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GUCA2B elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GUCA2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GUCA2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GUCA2B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GUCA2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 160298221
• NCBI GeneID: 14916
• NCBI Accession #: NP_032217.2
• NCBI GenBank Nucleotide #: NM_008191.2
• UniProt Accession #: O09051
• Molecular Weight: 11,628 Da
• NCBI Official Full Name: guanylate cyclase activator 2B preproprotein
• NCBI Official Synonym Full Names: guanylate cyclase activator 2b (retina)
• NCBI Official Symbol: Guca2b
• NCBI Official Synonym Symbols: Ugn; Gcap2; AV066530
• NCBI Protein Information: guanylate cyclase activator 2B; uroguanylin; granule cell antiserum positive 2
• UniProt Protein Name: Guanylate cyclase activator 2B
• Protein Family: Guanylate cyclase activator
• UniProt Gene Name: Guca2b
• UniProt Synonym Gene Names: UGN
• UniProt Entry Name: GUC2B_MOUSE

NCBI Description

This gene encodes a member of the guanylin family and preproprotein that is proteolytically processed to generate a mature protein product. The mature protein product, known as uroguanylin, is an endogenous ligand for the guanylate cyclase-C receptor and may regulate salt and water homeostasis in the intestine and kidneys. Homozygous knockout mice for this gene exhibit impaired sodium chloride excretion and elevated arterial pressure. This gene is present in a gene cluster with a related guanylin family member on chromosome 4. [provided by RefSeq, Sep 2015]

Uniprot Description

GUCA2B: Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport. Belongs to the guanylin family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: photoreceptor outer segment; extracellular region

Molecular Function: guanylate cyclase activator activity

Biological Process: cGMP biosynthetic process; regulation of cyclic nucleotide biosynthetic process; negative regulation of blood pressure; excretion; body fluid secretion