Mouse Talin-1 ELISA Kit | Tln1 elisa kit

Mouse Talin-1 ELISA Kit

Cat. Num. AAA288347

• Gene Names: Tln1; Tln
• Reactivity: Mouse
• Synonyms: Talin-1; Mouse Talin-1 ELISA Kit; Tln1 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse Talin-1. No significant cross-reactivity or interference was observed.
• Sequence Length: 2541

• Assay Type: Sandwich
• Detection Range: 0.312-20 ng/ml
• Sensitivity: 0.171 ng/mL
• Intra-Assay CV: <=5.7%
• Inter-Assay CV: <=8.2%
• Recovery: 112%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Tln1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Talin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Talin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Talin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Talin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 227116327
• NCBI GeneID: 21894
• NCBI Accession #: NP_035732.2
• NCBI GenBank Nucleotide #: NM_011602.5
• UniProt Accession #: P26039; Q8VEF0; A2AIM8
• Molecular Weight: 269,821 Da
• NCBI Official Full Name: talin-1
• NCBI Official Synonym Full Names: talin 1
• NCBI Official Symbol: Tln1
• NCBI Official Synonym Symbols: Tln
• NCBI Protein Information: talin-1
• UniProt Protein Name: Talin-1
• Protein Family: Talin
• UniProt Gene Name: Tln1
• UniProt Synonym Gene Names: Tln
• UniProt Entry Name: TLN1_MOUSE

Uniprot Description

Talin-1: a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Cellular Component: cell junction; cell projection; cytoplasm; cytoskeleton; focal adhesion; membrane; microtubule organizing center; plasma membrane; ruffle

Molecular Function: actin binding; actin filament binding; integrin binding; LIM domain binding; protein binding; protein complex binding; structural constituent of cytoskeleton; vinculin binding

Biological Process: cell adhesion; cell-substrate junction assembly; cortical actin cytoskeleton organization and biogenesis; cytoskeletal anchoring