Typical Testing Data/Standard Curve (for reference only)
Mouse Metalloproteinase inhibitor 2 ELISA Kit | Timp2 elisa kit
Mouse Metalloproteinase inhibitor 2 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Metalloproteinase inhibitor 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Metalloproteinase inhibitor 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Metalloproteinase inhibitor 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Metalloproteinase inhibitor 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family.
Protein type: Secreted; Extracellular matrix; Inhibitor; Secreted, signal peptide; Motility/polarity/chemotaxis; Cell development/differentiation
Cellular Component: basement membrane; cell soma; cell surface; extracellular region; extracellular space; growth cone; neuron projection
Molecular Function: enzyme activator activity; enzyme inhibitor activity; integrin binding; metal ion binding; metalloendopeptidase inhibitor activity; protease binding; protease inhibitor activity
Biological Process: negative regulation of cell proliferation; negative regulation of membrane protein ectodomain proteolysis; negative regulation of metalloenzyme activity; negative regulation of mitotic cell cycle; negative regulation of peptidase activity; negative regulation of proteolysis; negative regulation of Ras protein signal transduction; positive regulation of adenylate cyclase activity; positive regulation of MAPKKK cascade; positive regulation of neuron differentiation; regulation of cAMP metabolic process; regulation of MAPKKK cascade; regulation of neuron differentiation; regulation of Rap protein signal transduction; response to cytokine stimulus; response to hormone stimulus
Research Articles on Timp2
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Product Notes
The Mouse Timp2 timp2 (Catalog #AAA2882480) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2882480 ELISA Kit recognizes Mouse Timp2. It is sometimes possible for the material contained within the vial of "Metalloproteinase inhibitor 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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