Human Tyrosine-protein kinase receptor Tie-1 ELISA Kit | TIE1 elisa kit

Human Tyrosine-protein kinase receptor Tie-1 ELISA Kit

Cat. Num. AAA2885329

• Gene Names: TIE1; TIE; JTK14
• Reactivity: Human
• Synonyms: Tyrosine-protein kinase receptor Tie-1; Human Tyrosine-protein kinase receptor Tie-1 ELISA Kit; TIE1; TIE; 2.7.10.1; TIE1 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human Tyrosine-protein kinase receptor Tie-1
• Sequence Length: 1138

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: <0.079 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=5.9%
• Inter-assay Precision: Inter-Assay CV: <=9.7%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TIE1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4885631
• NCBI GeneID: 7075
• NCBI Accession #: NP_005415.1
• NCBI GenBank Nucleotide #: NM_005424.4
• UniProt Accession #: P35590; B5A949; B5A950
• Molecular Weight: 34,138 Da
• NCBI Official Full Name: tyrosine-protein kinase receptor Tie-1 isoform 1
• NCBI Official Synonym Full Names: tyrosine kinase with immunoglobulin like and EGF like domains 1
• NCBI Official Symbol: TIE1
• NCBI Official Synonym Symbols: TIE; JTK14
• NCBI Protein Information: tyrosine-protein kinase receptor Tie-1
• UniProt Protein Name: Tyrosine-protein kinase receptor Tie-1
• Protein Family: Tyrosine-protein kinase receptor
• UniProt Gene Name: TIE1
• UniProt Synonym Gene Names: TIE
• UniProt Entry Name: TIE1_HUMAN

NCBI Description

This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

TIE1: Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.

Protein type: Membrane protein, integral; Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; TK group; Tie family

Chromosomal Location of Human Ortholog: 1p34-p33

Cellular Component: integral to plasma membrane

Molecular Function: ATP binding; protein binding; transmembrane receptor protein tyrosine kinase activity

Biological Process: angiogenesis; in utero embryonic development; mesoderm development; negative regulation of angiogenesis; negative regulation of cell migration; peptidyl-tyrosine phosphorylation; plasma membrane fusion; response to retinoic acid; signal transduction; vasculogenesis