Testing Data
(Fig: Western blot analysis on K562 cell lysates using anti-Tie1 polyclonal antibody)
TIE-1 Polyclonal Antibody | anti-TIE-1 antibody
TIE-1 Antibody
Gene Names
TIE1; TIE; JTK14
Reactivity
Mouse, Rat, Human
Applications
Western Blot, Immunocytochemistry
Purity
Affinity purified
Synonyms
TIE-1; Polyclonal Antibody; TIE-1 Antibody; anti-TIE-1 antibody
Reactivity
Mouse, Rat, Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of N-terminal residues of Tie-1.
Purity/Purification
Affinity purified
Form/Format
1*TBS (pH7.4), 0.5%BSA, 25% Glycerol.
Sequence Length
1138
Applicable Applications for anti-TIE-1 antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
WB: 1:5,000
Preservative
0.05% Sodium Azide.
Cellular Localization
Cell Membrane
Positive Control
K562
Preparation and Storage
Store at +4 degree C after thawing. Aliquot store at-20 degree C or-80 degree C. Avoid repeated freeze/thaw cycles.
Testing Data
(Fig: Western blot analysis on K562 cell lysates using anti-Tie1 polyclonal antibody)
Testing Data
(Fig: Western blot analysis on K562 cell lysates using anti-Tie1 polyclonal antibody)
Related Product Information for anti-TIE-1 antibody
The Tie-1 receptor is an endothelial specific cell surface tyrosine kinase that is indispensable for endothelial functions. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Tie-1 and Tie-2 are receptor tyrosine kinases (RTKs) that are exclusively expressed in endothelial cells and play important roles in endothelial cell biology.
References
1. PuriMC, Rossant J, Alitalo K, et al.The receptor tyrosine kinase Tie is required for integrity and survival of Vascular endothelial cells[J].EMBO J, 1.95, 1.(5): 5884-5891. 2. Partanen J., Armstrong E. et al. "A novel endothelial cell surface receptor tyrosine kinase with extracellular epidermal growth factor homology domains." Mol. Cell. Biol. 1.:1.98-1.07(1.92.). 3. The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." Genome Res. 1.:2.1.2.7(2.04).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
125kDa
NCBI Official Full Name
tyrosine-protein kinase receptor Tie-1 isoform 1
NCBI Official Synonym Full Names
tyrosine kinase with immunoglobulin like and EGF like domains 1
NCBI Official Symbol
TIE1
NCBI Official Synonym Symbols
TIE; JTK14
NCBI Protein Information
tyrosine-protein kinase receptor Tie-1
UniProt Protein Name
Tyrosine-protein kinase receptor Tie-1
UniProt Gene Name
TIE1
UniProt Synonym Gene Names
TIE
NCBI Description
This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
Uniprot Description
Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
Research Articles on TIE-1
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Product Notes
The TIE-1 tie1 (Catalog #AAA856755) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The TIE-1 Antibody reacts with Mouse, Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's TIE-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). WB: 1:5,000. Researchers should empirically determine the suitability of the TIE-1 tie1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIE-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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