Human Peptide YY (PYY) ELISA Kit | PYY elisa kit

Human Peptide YY (PYY) ELISA Kit

Cat. Num. AAA2700455

• Gene Names: PYY; PYY1; PYY-I
• Reactivity: Human
• Synonyms: Peptide YY (PYY); Human Peptide YY (PYY) ELISA Kit; PYY1; Peptide tyrosine tyrosine; PYY elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Peptide YY (PYY).; No significant cross-reactivity or interference between Peptide YY (PYY) and analogues was observed.
• Sequence Length: 97

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 12.35-1,000pg/mL
• Sensitivity: < 4.77pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptide YY (PYY) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptide YY (PYY) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PYY elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Peptide YY (PYY) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Peptide YY (PYY) and unlabeled Peptide YY (PYY) (Standards or samples) with the pre-coated antibody specific to Peptide YY (PYY). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Peptide YY (PYY) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Peptide YY (PYY) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 300068956
• NCBI GeneID: 5697
• NCBI Accession #: NP_004151.3
• NCBI GenBank Nucleotide #: NM_004160.5
• UniProt Accession #: P10082; Q5U5Q6; Q6FGH8
• Molecular Weight: 10,360 Da
• NCBI Official Full Name: peptide YY preproprotein
• NCBI Official Synonym Full Names: peptide YY
• NCBI Official Symbol: PYY
• NCBI Official Synonym Symbols: PYY1; PYY-I
• NCBI Protein Information: peptide YY
• UniProt Protein Name: Peptide YY
• Protein Family: Peptide
• UniProt Gene Name: PYY
• UniProt Synonym Gene Names: PYY

NCBI Description

This gene encodes a member of the neuropeptide Y (NPY) family of peptides. The encoded preproprotein is proteolytically processed to generate two alternative peptide products that differ in length by three amino acids. These peptides, secreted by endocrine cells in the gut, exhibit different binding affinities for each of the neuropeptide Y receptors. Binding of the encoded peptides to these receptors mediates regulation of pancreatic secretion, gut mobility and energy homeostasis. Rare variations in this gene could increase susceptibility to obesity and elevated serum levels of the encoded peptides may be associated with anorexia nervosa. [provided by RefSeq, Feb 2016]

Uniprot Description

This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.