SDS-PAGE
Thermobifida Fusca Cutinase Recombinant Protein
Recombinant Thermobifida Fusca Cutinase Protein (C-His)
Purity
> 95% as determined by reducing SDS-PAGE.
Synonyms
Thermobifida Fusca Cutinase; Recombinant Thermobifida Fusca Cutinase Protein (C-His); Cutinase; Thermobifida Fusca Cutinase recombinant protein
Host
E Coli
Purity/Purification
> 95% as determined by reducing SDS-PAGE.
Form/Format
Supplied as a 0.2 um filtered solution of PBS, 50% Glycerol, pH7.4.
Concentration
1.0mg/mL (varies by lot)
Sequence
Ala1-Phe261
Species
Thermobifida Fusca
Endotoxin
< 1.0 EU per ug as determined by the LAL method.
Fusion Tag
C-His
Preparation and Storage
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for Thermobifida Fusca Cutinase recombinant protein
Protein Construction: Recombinant Thermobifida fusca Cutinase is produced by our E Coli expression system and the target gene encoding Ala1-Phe261 is expressed with a 6His tag at the C-terminus.
Background: Cutinase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is cutin hydrolase. Cutinase is a serine esterase containing the classical Ser, His, Asp triad of serine hydrolases. The protein belongs to the alpha-beta class, with a central beta-sheet of 5 parallel strands covered by 5 helices on either side of the sheet. Cutin monomers released from the cuticle by small amounts of cutinase on fungal spore surfaces can greatly increase the amount of cutinase secreted by the spore. The active site cleft is partly covered by 2 thin bridges formed by amino acid side chains, by contrast with the hydrophobic lid possessed by other lipases. The protein also contains 2 disulfide bridges, which are essential for activity, their cleavage resulting in complete loss of enzymatic activity.
Background: Cutinase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is cutin hydrolase. Cutinase is a serine esterase containing the classical Ser, His, Asp triad of serine hydrolases. The protein belongs to the alpha-beta class, with a central beta-sheet of 5 parallel strands covered by 5 helices on either side of the sheet. Cutin monomers released from the cuticle by small amounts of cutinase on fungal spore surfaces can greatly increase the amount of cutinase secreted by the spore. The active site cleft is partly covered by 2 thin bridges formed by amino acid side chains, by contrast with the hydrophobic lid possessed by other lipases. The protein also contains 2 disulfide bridges, which are essential for activity, their cleavage resulting in complete loss of enzymatic activity.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
Mol_Mass: 29.5 kDa
AP_Mol_Mass: 28-30 kDa
AP_Mol_Mass: 28-30 kDa
Similar Products
Product Notes
The Thermobifida Fusca Cutinase (Catalog #AAA2557618) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Ala1-Phe26 1. It is sometimes possible for the material contained within the vial of "Thermobifida Fusca Cutinase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
