Rabbit RPL15 Polyclonal Antibody | anti-RPL15 antibody
RPL15 Antibody (N-term)
Western Blot (WB)
(RPL15 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the RPL15 antibody detected the RPL15 protein (arrow).)
Western Blot (WB)
(RPL15 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the RPL15 antibody detected the RPL15 protein (arrow).)
Immunohistochemistry (IHC)
(RPL15 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RPL15 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(RPL15 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
consist of a small 40S subunit and a large 60S subunit. Together
these subunits are composed of 4 RNA species and approximately 80
structurally distinct proteins. This gene encodes a ribosomal
protein that is a component of the 60S subunit. The protein belongs
to the L15E family of ribosomal proteins. It is located in the
cytoplasm. This gene shares sequence similarity with the yeast
ribosomal protein YL10 gene. Although this gene has been referred
to as RPL10, its official symbol is RPL15. This gene has been shown
to be overexpressed in some esophageal tumors compared to normal
matched tissues. Transcript variants utilizing alternative polyA
signals exist. As is typical for genes encoding ribosomal proteins,
there are multiple processed pseudogenes of this gene dispersed
through the genome.
Wang, H., et al. BMC Cancer 6, 91 (2006) :
Andersen, J.S., et al. Nature 433(7021):77-83(2005)
Kapp, L.D., et al. Annu. Rev. Biochem. 73, 657-704 (2004) :
Mazumder, B., et al. Cell 115(2):187-198(2003)
NCBI and Uniprot Product Information
NCBI Description
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15E family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence similarity with the yeast ribosomal protein YL10 gene. Although this gene has been referred to as RPL10, its official symbol is RPL15. This gene has been shown to be overexpressed in some esophageal tumors compared to normal matched tissues. Alternate splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Nov 2011]
Uniprot Description
RPL15: a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15E family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence similarity with the yeast ribosomal protein YL10 gene. Although this gene has been referred to as RPL10, its official symbol is RPL15. This gene has been shown to be overexpressed in some esophageal tumors compared to normal matched tissues. Alternate splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Nov 2011]
Protein type: Ribosomal; Translation
Chromosomal Location of Human Ortholog: 3p24.2
Cellular Component: ribosome; cytosol; nucleus
Molecular Function: protein binding; structural constituent of ribosome; RNA binding
Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; viral reproduction; translation; translational initiation; mRNA catabolic process, nonsense-mediated decay; gene expression; viral transcription; translational termination; viral infectious cycle
Disease: Diamond-blackfan Anemia 12
Research Articles on RPL15
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Product Notes
The RPL15 rpl15 (Catalog #AAA9205521) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 21-50. The RPL15 Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: Bovine, Monkey, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's RPL15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the RPL15 rpl15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPL15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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