Typical Testing Data/Standard Curve (for reference only)
Mouse Tyrosine 3-monooxygenase ELISA Kit | Th elisa kit
Mouse Tyrosine 3-monooxygenase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
TH: an enzyme involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Four splice variant isoforms have been described.
Protein type: Vesicle; Amino Acid Metabolism - tyrosine; Endoplasmic reticulum; Mitochondrial; EC 1.14.16.2; Oxidoreductase
Cellular Component: axon; cell soma; cytoplasm; cytoplasmic vesicle; cytoplasmic vesicle membrane; cytosol; dendrite; internal side of plasma membrane; melanosome membrane; mitochondrion; neuron projection; nucleus; perikaryon; smooth endoplasmic reticulum; synaptic vesicle; terminal button
Molecular Function: amino acid binding; dopamine binding; enzyme binding; ferric iron binding; ferrous iron binding; iron ion binding; metal ion binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen; oxygen binding; protein domain specific binding; tyrosine 3-monooxygenase activity
Biological Process: aromatic amino acid family metabolic process; catecholamine biosynthetic process; dopamine biosynthetic process; dopamine biosynthetic process from tyrosine; eating behavior; embryonic camera-type eye morphogenesis; epinephrine biosynthetic process; eye photoreceptor cell development; heart development; learning; locomotory behavior; mating behavior; memory; neurotransmitter biosynthetic process; norepinephrine biosynthetic process; organ morphogenesis; regulation of heart contraction; response to ethanol; response to hypoxia; synaptic transmission, dopaminergic; synaptic vesicle amine transport; visual perception
Research Articles on Th
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Product Notes
The Mouse Th th (Catalog #AAA2886164) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2886164 ELISA Kit recognizes Mouse Th. It is sometimes possible for the material contained within the vial of "Tyrosine 3-monooxygenase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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